Abstract
Galectin-3 is involved in tumor cell proliferation, adhesion, angiogenesis and metastasis. Galectin-3 promotes β-catenin/Wnt signaling, and β-catenin-related oncogenesis has been frequently reported in osteosarcoma. However, the correlation between galectin-3 and β‑catenin signaling in osteosarcoma is poorly defined. We hypothesized that galectin-3 may control the migration and invasion of cancer cells and that silencing of galectin-3 would therefore, suppress motility in osteosarcoma cells. In the present study, we show that galectin-3 silencing in cultured human osteosarcoma cells had decreased cell migration and invasion capabilities; reduced the expression and activation of FAK, Src, Lyn, PI3K/Akt, ERK1/2 and β-catenin, which are key mediators of invasion; inhibited the expression and secretion of VEGF, MCP-1, IL-8, IL-6, MMP2/9 and phospho-Stat3; and potentiated sensitivity to cisplatin. Our results suggest that galectin-3 may be a feasible therapeutic target for osteosarcoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents / therapeutic use
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Bone Neoplasms / drug therapy
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Bone Neoplasms / genetics
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Bone Neoplasms / pathology*
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Cell Movement* / drug effects
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Cell Movement* / genetics
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Down-Regulation / drug effects
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Down-Regulation / genetics
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Drug Resistance, Neoplasm / drug effects
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Drug Resistance, Neoplasm / genetics
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Enzyme Activation / drug effects
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Enzyme Activation / genetics
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Focal Adhesion Kinase 1 / metabolism
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Galectin 3 / antagonists & inhibitors
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Galectin 3 / genetics*
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Gene Expression Regulation, Neoplastic / drug effects
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Gene Silencing*
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Humans
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Neoplasm Invasiveness
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Osteosarcoma / drug therapy
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Osteosarcoma / genetics
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Osteosarcoma / pathology*
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Tumor Cells, Cultured
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beta Catenin / genetics*
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beta Catenin / metabolism
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src-Family Kinases / metabolism
Substances
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Antineoplastic Agents
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CTNNB1 protein, human
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Galectin 3
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beta Catenin
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Focal Adhesion Kinase 1
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PTK2 protein, human
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lyn protein-tyrosine kinase
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src-Family Kinases