Abstract
The ARF GTPase Activating Protein 1 (ARFGAP1) associates mainly with the cytosolic side of Golgi cisternal membranes where it participates in the formation of both COPI and clathrin-coated vesicles. In this study, we show that ARFGAP1 associates transiently with lipid droplets upon addition of oleate in cultured cells. Also, that addition of cyclic AMP shifts ARFGAP1 from lipid droplets to the Golgi apparatus and that overexpression and knockdown of ARFGAP1 affect lipid droplet formation. Examination of human liver tissue reveals that ARFGAP1 is found associated with lipid droplets at steady state in some but not all hepatocytes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomarkers / metabolism
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Cell Line, Tumor
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Cyclic AMP / pharmacology
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Endoplasmic Reticulum / drug effects
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Endoplasmic Reticulum / metabolism
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Endoplasmic Reticulum / ultrastructure
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GTPase-Activating Proteins / metabolism*
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Gene Knockdown Techniques
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Golgi Apparatus / drug effects
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Golgi Apparatus / metabolism
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Golgi Apparatus / ultrastructure
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Hepatocytes / metabolism*
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Hepatocytes / ultrastructure
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Humans
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Lipid Droplets / drug effects
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Lipid Droplets / metabolism*
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Lipid Droplets / ultrastructure
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Liver / drug effects
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Liver / metabolism
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Oleic Acid / pharmacology
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Perilipin-3
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Protein Transport / drug effects
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Vesicular Transport Proteins / metabolism
Substances
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ARFGAP1 protein, human
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Biomarkers
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GTPase-Activating Proteins
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PLIN3 protein, human
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Perilipin-3
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Vesicular Transport Proteins
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Oleic Acid
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Cyclic AMP