Abstract
Dual rearrangement of immunoglobulin and T-cell antigen receptor (beta, delta) genes was demonstrated in a case of Philadelphia chromosome-positive chronic myeloid leukemia (CML) in blast crisis. The blast cells, showing L2 morphology and high activity of TdT, expressed pre-B cell (CD19+, Ia+) and myeloid (CD13+, CD34+) surface antigens but lacket T-cell antigens (CD2-, CD7-). Cytogenetic studies on bone marrow and peripheral blood revealed the Phl chromosome in all metaphases analyzed, majority of which also had the additional chromosome changes, +8, +10, +21. Furthermore, molecular analysis of the breakpoint cluster region (bcr) on chromosome 22 showed a rearrangement, confirming the CML origin of the blast cells.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Blast Crisis / enzymology
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Blast Crisis / genetics*
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Chromosomes, Human, Pair 10
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Chromosomes, Human, Pair 21
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Chromosomes, Human, Pair 8
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DNA Nucleotidylexotransferase / blood
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Gene Rearrangement, T-Lymphocyte*
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Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
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Genes, Immunoglobulin*
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Humans
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin delta-Chains / genetics
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Karyotyping
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / enzymology
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology
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Male
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Middle Aged
Substances
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Immunoglobulin Heavy Chains
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Immunoglobulin delta-Chains
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DNA Nucleotidylexotransferase