Overexpression of microRNA biogenesis machinery: Drosha, DGCR8 and Dicer in multiple sclerosis patients

J Clin Neurosci. 2015 Jan;22(1):200-3. doi: 10.1016/j.jocn.2014.06.106. Epub 2014 Oct 30.

Abstract

We aimed to evaluate the expression of the major components of microRNA biogenesis machinery including Drosha, Dicer and DiGeorge syndrome critical region gene 8 (DGCR8) in multiple sclerosis (MS) patients. The expression levels of these components in relapsing remitting multiple sclerosis (RRMS) patients were significantly up-regulated in comparison to healthy controls. DGCR8 was up-regulated 4.9 times in RRMS patients versus healthy controls, and Drosha was up-regulated 3.58 times. Additionally, the expression level of Dicer was 2.11 times higher in RRMS patients than the healthy controls. In conclusion, our results suggest that overexpression of Drosha, Dicer and DGCR8 may contribute to the pathogenesis of MS. Further investigation may introduce microRNA biogenesis machinery as MS markers and therapeutic targets.

Keywords: DGCR8; Dicer; Drosha; Gene expression; MicroRNA machinery; Multiple sclerosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • DEAD-box RNA Helicases / biosynthesis*
  • DEAD-box RNA Helicases / genetics*
  • DNA / biosynthesis
  • DNA / genetics
  • Disability Evaluation
  • Female
  • Humans
  • Male
  • MicroRNAs / biosynthesis*
  • MicroRNAs / genetics*
  • Multiple Sclerosis / genetics*
  • Multiple Sclerosis, Relapsing-Remitting / genetics
  • Polymerase Chain Reaction
  • RNA-Binding Proteins / biosynthesis*
  • RNA-Binding Proteins / genetics*
  • Ribonuclease III / biosynthesis*
  • Ribonuclease III / genetics*
  • Up-Regulation

Substances

  • DGCR8 protein, human
  • MicroRNAs
  • RNA-Binding Proteins
  • DNA
  • DICER1 protein, human
  • DROSHA protein, human
  • Ribonuclease III
  • DEAD-box RNA Helicases