miR-7-5p suppresses cell proliferation and induces apoptosis of breast cancer cells mainly by targeting REGγ

Cancer Lett. 2015 Mar 1;358(1):27-36. doi: 10.1016/j.canlet.2014.12.014. Epub 2014 Dec 12.

Abstract

Proteasome activator subunit 3 (REGγ) has a key role in breast cancer by promoting protein proteolysis, but methods to block REGγ expression remain elusive. In this study, we found that the expression of REGγ is significantly upregulated in breast cancer, and that the knockdown of REGγ expression suppresses cell proliferation and induces apoptosis in vitro. Furthermore, REGγ was identified as a direct downstream target of miR-7-5p, and there was an inverse correlation between the expression of REGγ and miR-7-5p. The overexpression of miR-7-5p inhibited cell proliferation and induced apoptosis by mainly targeting REGγ in vitro and in vivo. Our data indicate that miR-7-5p has a critical function through blocking REGγ in breast cancer cells.

Keywords: Breast cancer; Proliferation; REGγ; miR-7-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics*
  • Autoantigens / biosynthesis*
  • Autoantigens / genetics
  • Breast Neoplasms / genetics*
  • Breast Neoplasms / pathology
  • Cell Proliferation / genetics
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Knockdown Techniques
  • Humans
  • MCF-7 Cells
  • MicroRNAs / genetics*
  • Proteasome Endopeptidase Complex / biosynthesis*
  • Proteasome Endopeptidase Complex / genetics
  • Xenograft Model Antitumor Assays

Substances

  • Autoantigens
  • Ki antigen
  • MIRN7 microRNA, human
  • MicroRNAs
  • Proteasome Endopeptidase Complex