Abstract
This study aimed to investigate the effects of Se-enriched probiotics (SP) on the liver fibrosis induced by CCl4 in rats. The results showed that SP significantly decreased serum alanine aminotransferase (87.0 ± 1.96 U/L), aspartate aminotransferase (101 ± 3.13 U/L), hepatic hydroxyproline (898 ± 72.5 μg/g), and malondialdehyde (2.39 ± 0.34 nmol/mg) levels, but increased glutathione peroxidase (37.2 ± 3.19 U/mg), superoxide dismutase (201 ± 19.2 U/mg), and glutathione levels (3.32 ± 0.25 mg/g) (P < 0.05) in rats treated by CCl4. SP suppressed hepatic inflammation and necrosis induced by CCl4. Moreover, SP significantly reduced the expression of α-smooth muscle actin, collagen, TGF-β1, TIMP-1, and inflammation-related gene and induced apoptosis of activated hepatic stellate cells (P < 0.05) in rats treated by CCl4. Our results suggest that SP could protect the liver from fibrosis by attenuating hepatic oxidative stress, suppressing hepatic inflammation, and inducing apoptosis of hepatic stellate cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alanine Transaminase / genetics
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Alanine Transaminase / metabolism
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Animals
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Aspartate Aminotransferases / genetics
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Aspartate Aminotransferases / metabolism
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Carbon Tetrachloride / adverse effects
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Glutathione / metabolism
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Glutathione Peroxidase / genetics
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Glutathione Peroxidase / metabolism
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Humans
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Lactobacillus acidophilus / chemistry*
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Lactobacillus acidophilus / metabolism
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Liver Cirrhosis / chemically induced
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Liver Cirrhosis / genetics
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Liver Cirrhosis / metabolism
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Liver Cirrhosis / prevention & control*
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Male
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Malondialdehyde / metabolism
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Oxidative Stress / drug effects
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Probiotics / administration & dosage*
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Probiotics / analysis
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Probiotics / metabolism
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Protective Agents / administration & dosage*
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Protective Agents / metabolism
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Rats
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Rats, Wistar
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Selenium / administration & dosage*
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Selenium / analysis
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Selenium / metabolism
Substances
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Protective Agents
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Malondialdehyde
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Carbon Tetrachloride
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Glutathione Peroxidase
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Aspartate Aminotransferases
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Alanine Transaminase
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Glutathione
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Selenium