Abstract
Using iodinated insulin-like growth factors (IGFs) we have detected receptors for IGF-I at the cell surface of the clonally derived human embryonal carcinoma cell line Tera 2 clone 13. Affinity crosslinking of IGFs to Tera 2 clone 13-derived membrane preparations revealed the presence of proteins with features of both type-I and type-II IGF receptors. Treatment of Tera 2 clone 13 cells with retinoic acid to induce differentiation results in an increased number of cell surface receptors, apparently without altering the ratio of type-I and type-II receptors. In addition, Tera 2 clone 13 IGF-I receptors catalyze (auto)phosphorylation at tyrosine upon IGF-I and insulin binding. These findings suggest that type-I IGF receptors might be involved in mediating the effects of IGFs and insulin upon the proliferation of Tera 2 clone 13 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Cell Membrane / metabolism
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Cell Membrane / ultrastructure
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Cell Transformation, Neoplastic / drug effects
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Gene Expression
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Humans
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Insulin / pharmacology
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Insulin-Like Growth Factor I / pharmacology
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Insulin-Like Growth Factor II / pharmacology
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Neoplasms, Germ Cell and Embryonal / metabolism
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Neoplasms, Germ Cell and Embryonal / pathology
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Neoplasms, Germ Cell and Embryonal / ultrastructure*
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Phosphotransferases / metabolism
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / physiology*
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Receptors, Somatomedin
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Teratoma / metabolism
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Teratoma / pathology
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Teratoma / ultrastructure*
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Tretinoin / pharmacology
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Tumor Cells, Cultured / metabolism
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Tumor Cells, Cultured / pathology
Substances
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Insulin
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Receptors, Cell Surface
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Receptors, Somatomedin
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Tretinoin
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Insulin-Like Growth Factor I
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Insulin-Like Growth Factor II
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Phosphotransferases