Molecular cloning, sequence and functional expression of the cDNA for the human thyrotropin receptor

Biochem Biophys Res Commun. 1989 Dec 29;165(3):1184-90. doi: 10.1016/0006-291x(89)92727-7.

Abstract

We screened a human thyroid cDNA library with two synthetic oligonucleotides based on the reported amino acid sequence of the 3rd and 4th transmembrane domains of a putative human TSH receptor and related receptors. The nucleotide sequence of a 4 kb clone revealed an open reading frame of 764 amino acids (86,816 Daltons) with a putative signal peptide, seven transmembrane domains, five potential glycosylation sites, and a very short intracytoplasmic region. Homology with the extracellular domain of the pig LH/CG receptor was only 33%. Chinese hamster ovary cells stably transfected with this cDNA in an expression vector generated a functional receptor, able to activate adenylate cyclase, specifically in response to TSH stimulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular*
  • Codon
  • Cricetinae
  • Cyclic AMP / metabolism
  • DNA / genetics
  • Gene Expression*
  • Glycosylation
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Receptors, LH / genetics
  • Receptors, Thyrotropin / genetics*
  • Sequence Homology, Nucleic Acid
  • Transfection

Substances

  • Codon
  • Receptors, LH
  • Receptors, Thyrotropin
  • DNA
  • Cyclic AMP