MiR-204, down-regulated in retinoblastoma, regulates proliferation and invasion of human retinoblastoma cells by targeting CyclinD2 and MMP-9

FEBS Lett. 2015 Feb 27;589(5):645-50. doi: 10.1016/j.febslet.2015.01.030. Epub 2015 Jan 31.

Abstract

Aberrant expression of miR-204 had been frequently reported in cancer studies; however, the mechanism of its function in retinoblastoma remained unknown. Here, we reported that miR-204 was frequently downregulated in retinoblastoma tissues and cell lines. Enforced expression of miR-204 inhibited retinoblastoma cells' proliferation and invasion. In vivo study indicated that restoration of miR-204 inhibited tumor growth. CyclinD2 and MMP-9 were identified as potential targets of miR-204. In addition, a reverse correlation between miR-204 and CyclinD2 or MMP-9 expression was noted in retinoblastoma tissues. Taken together, our results identified a crucial tumor suppressive role of miR-204 in the progression of retinoblastoma.

Keywords: CyclinD2; MMP-9; Retinoblastoma; miR-204.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Proliferation / genetics
  • Cell Proliferation / physiology*
  • Cyclin D2 / genetics
  • Cyclin D2 / metabolism*
  • Humans
  • In Vitro Techniques
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinase 9 / metabolism*
  • Mice
  • Mice, Nude
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Neoplasm Invasiveness / genetics
  • Neoplasm Invasiveness / pathology*
  • Real-Time Polymerase Chain Reaction
  • Retinoblastoma / genetics
  • Retinoblastoma / metabolism*
  • Retinoblastoma / pathology*
  • Retinoblastoma / therapy

Substances

  • Cyclin D2
  • MIRN204 microRNA, human
  • MicroRNAs
  • Matrix Metalloproteinase 9