Abstract
To address the relationship of altered expression of double-strand break repair proteins Ku70 and p53 in clinical colorectal cancer (CRC), we examined the expression pattern of Ku70 and p53 by using fluorescent immunohistochemistry and real-time PCR assays in CRC and pericancerous samples from 152 Chinese patients. The results showed that down-expression pattern of both Ku70 and p53 coexisted in the CRC samples with significant correlating rate (R (2) = 0.9103; P < 0.001), and the down-expression of Ku70 and p53 was significantly associated with the advanced tumor node metastasis stage (Ku70: HR 3.453 in recurrence and 4.182 in survival, P < 0.001; P53: HR 3.114 in recurrence and 4.113 in survival, P < 0.001). The down-regulated Ku70 and p53 were associated with poor disease-free survival. Loss of Ku70 and p53 expression might serve as a biomarker of poor prognosis in CRC patients.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Nuclear / genetics
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Antigens, Nuclear / metabolism*
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism*
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Case-Control Studies
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Colorectal Neoplasms / metabolism*
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Colorectal Neoplasms / mortality
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Colorectal Neoplasms / pathology
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Female
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Follow-Up Studies
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Humans
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Immunoenzyme Techniques
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Ku Autoantigen
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Lymphatic Metastasis
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Male
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Middle Aged
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Neoplasm Recurrence, Local / metabolism*
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Neoplasm Recurrence, Local / mortality
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Neoplasm Recurrence, Local / pathology
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Neoplasm Staging
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Prognosis
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Reverse Transcriptase Polymerase Chain Reaction
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Survival Rate
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / metabolism*
Substances
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Antigens, Nuclear
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Biomarkers, Tumor
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DNA-Binding Proteins
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RNA, Messenger
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TP53 protein, human
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Tumor Suppressor Protein p53
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Xrcc6 protein, human
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Ku Autoantigen