Secreted pyruvate kinase M2 facilitates cell migration via PI3K/Akt and Wnt/β-catenin pathway in colon cancer cells

Peng Yang; Zongwei Li; Yingying Wang; Lichao Zhang; Haili Wu; Zhuoyu Li

doi:10.1016/j.bbrc.2015.02.112

Secreted pyruvate kinase M2 facilitates cell migration via PI3K/Akt and Wnt/β-catenin pathway in colon cancer cells

Biochem Biophys Res Commun. 2015 Apr 3;459(2):327-332. doi: 10.1016/j.bbrc.2015.02.112. Epub 2015 Feb 28.

Authors

Peng Yang¹, Zongwei Li¹, Yingying Wang¹, Lichao Zhang¹, Haili Wu¹, Zhuoyu Li²

Affiliations

¹ Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, China.
² Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Shanxi University, Taiyuan 030006, China; College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. Electronic address: lzy@sxu.edu.cn.

PMID: 25732087
DOI: 10.1016/j.bbrc.2015.02.112

Abstract

Pyruvate Kinase M2 (PKM2) is a key glycolytic enzyme, which highly expressed in tumor cells, and plays a pivotal role in the growth, survival and metabolism reprogramming of cancer cells. Besides the location of cytoplasm as a glycolytic enzyme and the location of nucleus as a protein kinase, extracellular PKM2 is present in serum and feces of tumor patients. However, little is known about the secretion of PKM2 and its significance in the progression of colon cancer. Here we demonstrated that PKM2 could be secreted from colon cancer cells, and purified PKM2 protein mimicing the secreted PKM2 was able to promote colon cancer cell migration. Moreover, PI3K/Akt and Wnt/β-catenin signaling were involved in secreted PKM2 induced colon cancer cell migration. The results reveal critical roles of secreted PKM2 in the progression of colon cancer, and indicate that PKM2 may be a therapeutic target for colon cancer.

Keywords: Colon cancer; Migration; PKM1; PKM2; Secretion.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell Movement / physiology
Colonic Neoplasms / genetics
Colonic Neoplasms / metabolism*
Colonic Neoplasms / pathology
Gene Knockdown Techniques
HCT116 Cells
HT29 Cells
HeLa Cells
Humans
Phosphatidylinositol 3-Kinases / metabolism*
Proto-Oncogene Proteins c-akt / metabolism*
Pyruvate Kinase / antagonists & inhibitors
Pyruvate Kinase / metabolism*
RNA, Small Interfering / genetics
Signal Transduction
Wnt Signaling Pathway*
beta Catenin / antagonists & inhibitors
beta Catenin / genetics
beta Catenin / metabolism*

Substances

CTNNB1 protein, human
RNA, Small Interfering
beta Catenin
Phosphatidylinositol 3-Kinases
Pyruvate Kinase
Proto-Oncogene Proteins c-akt