Molecular monitoring of chronic myeloid leukemia: principles and interlaboratory standardization

Ann Hematol. 2015 Apr:94 Suppl 2:S219-25. doi: 10.1007/s00277-015-2315-1. Epub 2015 Mar 27.

Abstract

Serial quantification of BCR-ABL1 messenger RNA (mRNA) is an important therapeutic indicator for patients with chronic myeloid leukemia, but historically, there has been substantial variation in results reported by different laboratories. To help improve the comparability of results, an international scale (IS) for BCR-ABL1 was proposed which is being implemented by testing laboratories worldwide. This is being achieved most commonly by the derivation of laboratory-specific conversion factors, but increasingly by the use of kits or reagents that are calibrated to the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL1 mRNA. Recent attention has focused on the need to define and validate levels of deeper molecular response (MR) within the context of the IS. While there has been substantial progress in the alignment of results, BCR-ABL1 measurement is technically challenging and standardization is an ongoing process.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Antineoplastic Agents / adverse effects
  • Antineoplastic Agents / therapeutic use*
  • Biomarkers / blood
  • Biomarkers / metabolism
  • Bone Marrow / metabolism
  • Calibration
  • Drug Monitoring
  • Fusion Proteins, bcr-abl / antagonists & inhibitors*
  • Fusion Proteins, bcr-abl / genetics
  • Fusion Proteins, bcr-abl / metabolism
  • Humans
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / diagnosis*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / drug therapy
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / enzymology
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
  • Molecular Diagnostic Techniques*
  • Molecular Targeted Therapy* / adverse effects
  • Prognosis
  • Protein Kinase Inhibitors / adverse effects
  • Protein Kinase Inhibitors / therapeutic use*
  • RNA, Messenger / blood
  • RNA, Messenger / metabolism*
  • Real-Time Polymerase Chain Reaction
  • Reference Standards

Substances

  • Antineoplastic Agents
  • BCR-ABL1 fusion protein, human
  • Biomarkers
  • Protein Kinase Inhibitors
  • RNA, Messenger
  • Fusion Proteins, bcr-abl