Gene expression profiles of primary retinal pigment epithelial cells from apolipoprotein E knockout and human apolipoprotein E2 transgenic mice

D H Jo; J H Lee; H J Jun; J Kim; Q Wen; M H Hoang; Y S Yu; J H Kim; S J Lee

doi:10.4238/2015.March.13.14

Gene expression profiles of primary retinal pigment epithelial cells from apolipoprotein E knockout and human apolipoprotein E2 transgenic mice

Genet Mol Res. 2015 Mar 13;14(1):1855-67. doi: 10.4238/2015.March.13.14.

Authors

D H Jo¹, J H Lee², H J Jun², J Kim¹, Q Wen², M H Hoang², Y S Yu¹, J H Kim¹, S J Lee³

Affiliations

¹ Fight Against Angiogenesis-Related Blindness Laboratory, Clinical Research Institute, Seoul National University Hospital, Seoul, Republic of Korea.
² Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Institute of Biomedical Science and Safety, Korea University, Seoul, Republic of Korea.
³ Division of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Institute of Biomedical Science and Safety, Korea University, Seoul, Republic of Korea junelee@korea.ac.kr.

PMID: 25867331
DOI: 10.4238/2015.March.13.14

Abstract

Age-related macular degeneration (AMD) causes visual impairment in the elderly. In non-neovascular AMD, studies involving human subjects have suggested potential involvement of aberrant lipid metabolism. However, there have been no reports on gene expression patterns in animal models of non-neovascular AMD with abnormal lipid metabolism such as apolipoprotein E knockout and human apolipoprotein E2 transgenic mice. Transcriptome analysis was performed using retinal pigment epithelium cells of apoE knockout and apolipoprotein E2 mice using microarray analysis. C57BL/6, Rxrb, Pparbp, Vldlr, and Edf1, which are primarily related to lipid metabolism, were upregulated, while Tgfbr1 and Pdgfb, which are related to pathologic angiogenesis in AMD, were downregulated in both types of mice. Apolipoprotein E knockout and apolipoprotein E2 mice showed characteristic gene expression patterns in the transcriptome analysis of primary retinal pigment epithelium cells. These results suggest that specific genes associated with lipid metabolism and angiogenesis are involved in the pathogenesis and progression of AMD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Animals
Apolipoprotein E2 / genetics*
Apolipoproteins E / genetics
Calmodulin-Binding Proteins / genetics
Calmodulin-Binding Proteins / metabolism
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Disease Models, Animal
Down-Regulation
Epithelial Cells / metabolism*
Humans
Lipid Metabolism
Lymphokines / genetics
Lymphokines / metabolism
Macular Degeneration / genetics
Mice
Mice, Inbred C57BL
Mice, Knockout
Mice, Transgenic
Microarray Analysis
PPAR-beta / genetics
PPAR-beta / metabolism
Platelet-Derived Growth Factor / genetics
Platelet-Derived Growth Factor / metabolism
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism
Receptor, Transforming Growth Factor-beta Type I
Receptors, LDL / genetics
Receptors, LDL / metabolism
Receptors, Transforming Growth Factor beta / genetics
Receptors, Transforming Growth Factor beta / metabolism
Retinal Pigment Epithelium / cytology*
Transcriptome*
Up-Regulation

Substances

Apolipoprotein E2
Apolipoproteins E
Calmodulin-Binding Proteins
DNA-Binding Proteins
Edf1 protein, mouse
Lymphokines
PPAR-beta
Pdgfd protein, mouse
Platelet-Derived Growth Factor
Receptors, LDL
Receptors, Transforming Growth Factor beta
Rxrb protein, mouse
VLDL receptor
Protein Serine-Threonine Kinases
Receptor, Transforming Growth Factor-beta Type I
TGFBR1 protein, human
Tgfbr1 protein, mouse

Supplementary concepts

Macular Degeneration, Age-Related, 2