Downregulated Long Noncoding RNA BANCR Promotes the Proliferation of Colorectal Cancer Cells via Downregualtion of p21 Expression

Yongguo Shi; Yangchen Liu; Jirong Wang; Ding Jie; Tian Yun; Wang Li; Lin Yan; Keming Wang; Jifeng Feng

doi:10.1371/journal.pone.0122679

Downregulated Long Noncoding RNA BANCR Promotes the Proliferation of Colorectal Cancer Cells via Downregualtion of p21 Expression

PLoS One. 2015 Apr 30;10(4):e0122679. doi: 10.1371/journal.pone.0122679. eCollection 2015.

Authors

Yongguo Shi¹, Yangchen Liu², Jirong Wang³, Ding Jie³, Tian Yun³, Wang Li³, Lin Yan³, Keming Wang³, Jifeng Feng⁴

Affiliations

¹ Department of Oncology, Second Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, PR China; Taixing People's Hospital, Taixing, Jiangsu, PR China.
² Taixing People's Hospital, Taixing, Jiangsu, PR China.
³ Department of Oncology, Second Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, PR China.
⁴ Cancer Hospital of Jiangsu Province, Nanjing, Jiangsu, PR China.

Abstract

BRAF activated non-coding RNA (BANCR), a long non-coding RNA (lncRNA), is crucial for cell migration in melanoma cells and non-small cell lung cancer (NSCLC) cells. However, little is known regarding the role of this gene in the proliferation of colorectal cancer. Therefore, we investigated the involvement of BANCR in the proliferation of colorectal cancer cells. In this study, we show that BANCR expression was significantly down-regulated in colorectal cancer tissues compared with normal tissues, and overexpression of BANCR suppressed colorectal cancer cell growth in vitro and in vivo. We also determined that pCDNA-BANCR-mediated colorectal cancer cell proliferation was associated with induction of G0/G1 cell-cycle arrest and apoptosis enhancement through regulation of p21, and its effects were most likely posttranscriptional. Taken together, our findings suggest that down-regulation of BANCR contributes to the proliferation of colorectal cancer cells, at least in part, through the regulation of p21 protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Aged, 80 and over
Cell Line, Tumor
Colorectal Neoplasms / genetics
Colorectal Neoplasms / metabolism*
Colorectal Neoplasms / pathology
Cyclin-Dependent Kinase Inhibitor p21 / biosynthesis*
Cyclin-Dependent Kinase Inhibitor p21 / genetics
Down-Regulation
Female
G1 Phase Cell Cycle Checkpoints*
Gene Expression Regulation, Neoplastic*
Humans
Male
Middle Aged
Neoplasm Proteins / biosynthesis*
Neoplasm Proteins / genetics
RNA, Long Noncoding / genetics
RNA, Long Noncoding / metabolism*
RNA, Neoplasm / genetics
RNA, Neoplasm / metabolism*
Resting Phase, Cell Cycle*

Substances

BANCR long non-coding RNA, human
CDKN1A protein, human
Cyclin-Dependent Kinase Inhibitor p21
Neoplasm Proteins
RNA, Long Noncoding
RNA, Neoplasm

Grants and funding

This work was supported by the Outstanding Medical Academic Leader program of Jiangsu province (LG201126), the Six talents peak project of Jiangsu province (WSN-050), and the Medical Science and Technology Development Fund Project of Nanjing (YKK13178).