Human Surfactant Proteins A2 (SP-A2) and B (SP-B) Genes as Determinants of Respiratory Distress Syndrome

Indian Pediatr. 2015 May;52(5):391-4. doi: 10.1007/s13312-015-0643-9.

Abstract

Objective: To study the relationship between SP-A2 and SP-B gene polymorphisms and respiratory distress syndrome in preterm neonates.

Design: Cross-sectional.

Setting: Neonatal intensive care unit and the Molecular Biology unit of the Chemical Pathology Department, Kasr Alainy hospital, Cairo University.

Participants: Sixty-five preterm infants with respiratory distress syndrome and 50 controls. The genomic DNA was isolated using DNA extraction kits. SYBR Green-based real-time PCR was used to determine the variant genotypes of SP-A2 c.751 G>A and SP-B c.8714 G>C single nucleotide polymorphisms.

Results: Homozygosity of SP-A (OR 46, 95% CI 14-151) and SP-B (OR 5.2, 95% CI 2.3-11.4) alleles increased the risk of respiratory distress syndrome. The logistic regression model showed that genotypes SP-A2 (OR 164) and SP-B (OR 18) were directly related to the occurrence of respiratory distress syndrome, whereas gestational age (OR 0.57) and 5-minute Apgar score (OR 0.19) were inversely related to its occurrence.

Conclusions: There is a possible involvement of SP-A2 and SP-B genes polymorphisms in the genetic predisposition to respiratory distress syndrome.

MeSH terms

  • Cross-Sectional Studies
  • Egypt / epidemiology
  • Female
  • Genetic Predisposition to Disease / genetics*
  • Humans
  • Infant, Newborn
  • Polymorphism, Single Nucleotide / genetics
  • Pregnancy
  • Pulmonary Surfactant-Associated Protein A / genetics*
  • Pulmonary Surfactant-Associated Protein B / genetics*
  • Respiratory Distress Syndrome, Newborn / epidemiology
  • Respiratory Distress Syndrome, Newborn / genetics*

Substances

  • Pulmonary Surfactant-Associated Protein A
  • Pulmonary Surfactant-Associated Protein B
  • SFTPA2 protein, human