miR-362-5p inhibits proliferation and migration of neuroblastoma cells by targeting phosphatidylinositol 3-kinase-C2β

FEBS Lett. 2015 Jul 8;589(15):1911-9. doi: 10.1016/j.febslet.2015.05.056. Epub 2015 Jun 11.

Abstract

miR-362-5p is down-regulated in high-risk neuroblastoma and can function as a tumor suppressor. However, its role remains poorly understood. We show that miR-362-5p is down-regulated in metastatic neuroblastoma compared with primary neuroblastoma. Overexpression of miR-362-5p inhibits cell proliferation, migration and invasion of neuroblastoma cells in vitro and suppresses tumor growth of neuroblastoma in vivo. Phosphatidylinositol 3-kinase (PI3K)-C2β is a target of miR-362-5p. Knockdown of PI3K-C2β by siRNA had a similar effect to overexpression of miR-362-5p on SH-SY5Y cells. Overexpression of PI3K-C2β partially reversed tumor-suppressive effects of miR-362-5p. We suggest that miR-362-5p suppresses neuroblastoma cell growth and motility, partially by targeting PI3K-C2β.

Keywords: Motility; Neuroblastoma; Phosphatidylinositol 3-kinase; Proliferation; miR-362-5p.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / physiology
  • Base Sequence
  • Cell Line, Tumor
  • Cell Movement / physiology*
  • Cell Proliferation / physiology*
  • Class II Phosphatidylinositol 3-Kinases / genetics
  • Class II Phosphatidylinositol 3-Kinases / metabolism*
  • DNA Primers
  • Humans
  • MicroRNAs / physiology*
  • Neuroblastoma / enzymology
  • Neuroblastoma / pathology*
  • RNA, Small Interfering / genetics
  • Real-Time Polymerase Chain Reaction

Substances

  • DNA Primers
  • MIRN362 microRNA, human
  • MicroRNAs
  • RNA, Small Interfering
  • Class II Phosphatidylinositol 3-Kinases