Abstract
Hydrogen sulfide (H2S) can be synthesized in mammalian cells by cystathionine γ-lyase (CSE) and/or cystathionine β-synthase (CBS). Both CSE and CBS are expressed in rat gastric tissues but their role in human gastric neoplasia has been unclear. The aims of the present study were to detect CSE and CBS proteins in human gastric cancer and determine the effect of exogenous NaHS on the proliferation of gastric cancer cells. We found that both CSE and CBS proteins were expressed in human gastric cancer cells and upregulated in human gastric carcinoma mucosa compared with those in noncancerous gastric samples. NaHS induced apoptosis of gastric cancer cells by regulating apoptosis related proteins. Also, NaHS inhibited cancer cell migration and invasion. An antigastric cancer role of H2S is thus indicated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Antineoplastic Agents / toxicity*
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Apoptosis / drug effects*
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Caspase 3 / metabolism
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Cell Cycle Proteins / metabolism
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Cell Line, Tumor
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Cell Movement / drug effects
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Cystathionine beta-Synthase / genetics
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Cystathionine beta-Synthase / metabolism
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Cystathionine gamma-Lyase / genetics
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Cystathionine gamma-Lyase / metabolism
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Cytochromes c / metabolism
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Female
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Gastric Mucosa / metabolism
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Humans
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Hydrogen Sulfide / toxicity*
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Male
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Microscopy, Fluorescence
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Middle Aged
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Real-Time Polymerase Chain Reaction
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Stomach Neoplasms / metabolism
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Stomach Neoplasms / pathology*
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Up-Regulation
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bcl-2-Associated X Protein / metabolism
Substances
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Antineoplastic Agents
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Cell Cycle Proteins
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bcl-2-Associated X Protein
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Cytochromes c
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Caspase 3
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Cystathionine beta-Synthase
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Cystathionine gamma-Lyase
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Hydrogen Sulfide