Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis

Anthony Troegeler; Geanncarlo Lugo-Villarino; Søren Hansen; Voahangy Rasolofo; Maiken Lumby Henriksen; Kenichiro Mori; Katsuki Ohtani; Carine Duval; Ingrid Mercier; Alan Bénard; Jérome Nigou; Denis Hudrisier; Nobutaka Wakamiya; Olivier Neyrolles

doi:10.1371/journal.pone.0132692

Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis

PLoS One. 2015 Jul 14;10(7):e0132692. doi: 10.1371/journal.pone.0132692. eCollection 2015.

Affiliations

¹ Centre National de la Recherche Scientifique, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France; Université de Toulouse, Université Paul Sabatier, Institut de Pharmacologie et de Biologie Structurale, Toulouse, France.
² Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark.
³ Mycobacteria Unit, Pasteur Institute in Antananarivo, Antananarivo, Madagascar.
⁴ Department of Microbiology & Immunochemistry, Asahikawa Medical University, Asahikawa, Japan.

Abstract

Understanding the molecular components of immune recognition of the tuberculosis (TB) bacillus, Mycobacterium tuberculosis, can help designing novel strategies to combat TB. Here, we identify collectin CL-LK as a novel soluble C-type lectin able to bind M. tuberculosis, and characterize mycobacterial mannose-capped lipoarabinomannan as a primary ligand for CL-LK. Mice deficient in CL-K1, one of the CL-LK subunits, do not display altered susceptibility to M. tuberculosis. However, we found that the amount of CL-LK in the serum of patients with active TB is reduced, compared to that in controls, and correlates inversely to the magnitude of the immune response to the pathogen. These findings indicate that CL-LK might be of interest for future diagnostic and treatment monitoring purposes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Case-Control Studies
Collectins / blood
Collectins / deficiency
Collectins / genetics
Collectins / immunology*
Female
Humans
In Vitro Techniques
Ligands
Lipopolysaccharides / immunology
Lipopolysaccharides / metabolism
Mice
Mice, Inbred C57BL
Mice, Knockout
Mycobacterium tuberculosis / immunology*
Mycobacterium tuberculosis / pathogenicity
Receptors, Pattern Recognition / metabolism*
Tuberculosis, Pulmonary / blood
Tuberculosis, Pulmonary / immunology

Substances

Colec11 protein, human
Collectins
Ligands
Lipopolysaccharides
Receptors, Pattern Recognition
lipoarabinomannan

Grants and funding

This work was supported by Centre National de la Recherche Scientifique (CNRS), University of Toulouse, Agence Nationale de la Recherche (ANR)/Programme d’Investissements d’Avenir (PIA grant number ANR-11-EQUIPEX-0003) and ANR-12-BSV3-0002 B-TB grant, French Ministry of Higher Education and Research (fellowship to A.T.), Fondation pour la Recherche Médicale (FRM fellowships to G.L.V., A.B. and C.D.), European Union (NEWTBVAC project, project n° 241745), and Fondation Bettencourt-Schueller. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.