Abstract
MicroRNAs (miRNAs) are involved in human cancer including non-small cell lung cancer (NSCLC). In this study, we compared miRNA expression microarray of SPC-A-1sci (high metastatic) and SPC-A-1 (weakly metastatic) cells. We found that miRNA-10a was up-regulated in NSCLC compared with corresponding normal tissues. High expression of miR-10a was associated with tumor node metastasis and lymph node metastasis. Furthermore, overexpression of miR-10a promoted NSCLC cell proliferation, migration and invasion in vitro. We found that PTEN was a direct target of miR-10a in NSCLC. Also miR-10a activated the PTEN/AKT/ERK pathway. We suggest that miR-10a contributes to NSCLC by targeting PTEN.
Keywords:
NSCLC; PTEN; metastasis; microRNA-10a.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Carcinoma, Non-Small-Cell Lung / enzymology*
-
Carcinoma, Non-Small-Cell Lung / genetics
-
Carcinoma, Non-Small-Cell Lung / secondary
-
Cell Division
-
Cell Line, Tumor
-
Cell Movement
-
Cell Proliferation
-
Extracellular Signal-Regulated MAP Kinases / metabolism
-
Female
-
G2 Phase
-
Gene Expression Regulation, Enzymologic
-
Gene Expression Regulation, Neoplastic
-
Humans
-
Lung Neoplasms / enzymology*
-
Lung Neoplasms / genetics
-
Lung Neoplasms / pathology
-
Lymphatic Metastasis
-
Male
-
MicroRNAs / genetics
-
MicroRNAs / metabolism*
-
Middle Aged
-
Neoplasm Invasiveness
-
PTEN Phosphohydrolase / genetics
-
PTEN Phosphohydrolase / metabolism*
-
Proto-Oncogene Proteins c-akt / metabolism
-
RNA Interference
-
Signal Transduction
-
Transfection
-
Up-Regulation
Substances
-
MIRN10 microRNA, human
-
MicroRNAs
-
Proto-Oncogene Proteins c-akt
-
Extracellular Signal-Regulated MAP Kinases
-
PTEN Phosphohydrolase
-
PTEN protein, human