Ack1 overexpression promotes metastasis and indicates poor prognosis of hepatocellular carcinoma

Xiong Lei; Yun-Feng Li; Guo-Dong Chen; Di-Peng Ou; Xiao-Xin Qiu; Chao-Hui Zuo; Lian-Yue Yang

doi:10.18632/oncotarget.5872

Ack1 overexpression promotes metastasis and indicates poor prognosis of hepatocellular carcinoma

Oncotarget. 2015 Dec 1;6(38):40622-41. doi: 10.18632/oncotarget.5872.

Authors

Xiong Lei¹, Yun-Feng Li¹, Guo-Dong Chen¹, Di-Peng Ou², Xiao-Xin Qiu³, Chao-Hui Zuo³, Lian-Yue Yang^{1

2}

Affiliations

¹ Liver Cancer Laboratory, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
² Department of Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
³ Department of Abdominal Surgical Oncology, Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, Hunan, China.

Abstract

Despite the substantial data supporting the oncogenic role of Ack1, the predictive value and biologic role of Ack1 in hepatocellular carcinoma (HCC) metastasis remains unknown. In this study, both correlations of Ack1 expression with prognosis of HCC, and the role of Ack1 in metastasis of HCC were investigated in vitro and in vivo. Our results showed that Ack1 was overexpressed in human HCC tissues and cell lines. High Ack1 expression was associated with HCC metastasis and determined as a significant and independent prognostic factor for HCC after liver resection. Ack1 promoted HCC invasion and metastasis in vitro and in vivo. Mechanistically, we confirmed that Ack1 enhanced invasion and metastasis of HCC via EMT by mediating AKT phosphorylation. In conclusion, our study shows Ack1 is a novel prognostic biomarker for HCC and promotes metastasis of HCC via EMT by activating AKT signaling.

Keywords: Ack1; hepatocellular carcinoma; metastasis; prognosis.

Publication types

Randomized Controlled Trial

MeSH terms

Animals
Apoptosis
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Blotting, Western
Carcinoma, Hepatocellular / genetics
Carcinoma, Hepatocellular / metabolism
Carcinoma, Hepatocellular / secondary*
Case-Control Studies
Cell Adhesion
Cell Movement*
Cell Proliferation
Cohort Studies
Female
Follow-Up Studies
Gene Expression Regulation, Neoplastic
Humans
Immunoenzyme Techniques
Liver / metabolism
Liver / pathology
Liver Neoplasms / genetics
Liver Neoplasms / metabolism
Liver Neoplasms / pathology*
Lymphatic Metastasis
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Neoplasm Grading
Neoplasm Invasiveness
Neoplasm Recurrence, Local / genetics
Neoplasm Recurrence, Local / metabolism
Neoplasm Recurrence, Local / pathology*
Neoplasm Staging
Prognosis
Protein-Tyrosine Kinases / genetics
Protein-Tyrosine Kinases / metabolism*
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
Tumor Cells, Cultured
Xenograft Model Antitumor Assays

Substances

Biomarkers, Tumor
RNA, Messenger
Protein-Tyrosine Kinases
TNK2 protein, human