Glycoprotein isolated from Styrax japonica Siebold et al. Zuccarini inhibits oxidative and pro-inflammatory responses in HCT116 colonic epithelial cells and dextran sulfate sodium-treated ICR mice

Sei-Jung Lee; Jin Lee; Sooyeon Song; Kye-Taek Lim

doi:10.1016/j.fct.2015.11.004

Glycoprotein isolated from Styrax japonica Siebold et al. Zuccarini inhibits oxidative and pro-inflammatory responses in HCT116 colonic epithelial cells and dextran sulfate sodium-treated ICR mice

Food Chem Toxicol. 2016 Jan:87:12-22. doi: 10.1016/j.fct.2015.11.004. Epub 2015 Nov 26.

Authors

Sei-Jung Lee¹, Jin Lee², Sooyeon Song³, Kye-Taek Lim⁴

Affiliations

¹ Department of Veterinary Physiology, College of Veterinary Medicine, Research Institute for Veterinary Science, and BK21 PLUS Creative Veterinary Research Center, Seoul National University, Seoul 08826, South Korea.
² Department of Pathology, School of Medicine, University of California at San Diego La Jolla, CA 92093-0864, USA.
³ Division of Animal Science, Chonnam National University, 77 Yongbong-ro, Gwangju 61186, South Korea.
⁴ Division of Animal Science, Chonnam National University, 77 Yongbong-ro, Gwangju 61186, South Korea. Electronic address: ktlim@chonnam.ac.kr.

PMID: 26631293
DOI: 10.1016/j.fct.2015.11.004

Abstract

This study was carried out to investigate the anti-inflammatory potentials of a 38 kDa glycoprotein isolated from Styrax japonica Siebold et al Zuccarini (SJSZ glycoprotein). We found that SJSZ glycoprotein has concentration-dependent scavenging activity against DPPH and hydroxyl radicals in the cell-free systems. In colonic epithelial cells (HCT116 cells), the results showed that SJSZ glycoprotein inhibits the production of reactive oxygen species (ROS) induced by glucose/glucose oxidase (G/GO) in a concentration-dependent manner. Experimental mouse colitis was induced by adding dextran sulfate sodium (DSS) to the drinking water at a concentration of 4% (w/v) for 7 days. We figured out that administration of SJSZ glycoprotein (10 mg/kg) lowers the levels of disease activity index, myeloperoxidase activity, and histological inflammation in DSS-treated mice. In addition, SJSZ glycoprotein inhibited plasmic thiobarbituric acid reactive substances (TBARS) formation, nitric oxide (NO) production, and lactate dehydrogenase (LDH) release, accompanying the inhibition of colonic inflammatory signal proteins (NF-κB, iNOS, and COX-2) and inflammation-related cytokines (IL-1β, IL-6, and TNF-α). These results indicate that SJSZ glycoprotein inhibits oxidative and pro-inflammatory responses in mouse colitis.

Keywords: Colonic epithelial cells; Dextran sodium sulfate; Hydroxyl radicals; Mouse colitis; SJSZ glycoprotein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Colitis / chemically induced*
Colitis / drug therapy
Cyclooxygenase 2 / genetics
Cyclooxygenase 2 / metabolism
Dextran Sulfate / toxicity*
Gene Expression Regulation / drug effects
Glycoproteins / chemistry
Glycoproteins / pharmacology*
HCT116 Cells
Humans
Inflammation / drug therapy*
L-Lactate Dehydrogenase / blood
L-Lactate Dehydrogenase / metabolism
Male
Mice
Mice, Inbred ICR
NF-kappa B / genetics
NF-kappa B / metabolism
Nitric Oxide / blood
Nitric Oxide / metabolism
Nitric Oxide Synthase Type II / genetics
Nitric Oxide Synthase Type II / metabolism
Oxidative Stress / drug effects*
Reactive Oxygen Species
Styrax / chemistry*
Thiobarbituric Acid Reactive Substances / metabolism

Substances

Glycoproteins
NF-kappa B
Reactive Oxygen Species
Thiobarbituric Acid Reactive Substances
Nitric Oxide
Dextran Sulfate
L-Lactate Dehydrogenase
Nitric Oxide Synthase Type II
Cyclooxygenase 2