Association of the XPD and XRCC3 gene polymorphisms with oral squamous cell carcinoma in a Northeastern Brazilian population: A pilot study

Joabe dos Santos Pereira; Fabrícia Lima Fontes; Silvia Regina Batistuzzo de Medeiros; Roseana de Almeida Freitas; Lélia Batista de Souza; Márcia Cristina da Costa Miguel

doi:10.1016/j.archoralbio.2015.12.004

Association of the XPD and XRCC3 gene polymorphisms with oral squamous cell carcinoma in a Northeastern Brazilian population: A pilot study

Arch Oral Biol. 2016 Apr:64:19-23. doi: 10.1016/j.archoralbio.2015.12.004. Epub 2015 Dec 22.

Authors

Joabe dos Santos Pereira¹, Fabrícia Lima Fontes², Silvia Regina Batistuzzo de Medeiros², Roseana de Almeida Freitas³, Lélia Batista de Souza³, Márcia Cristina da Costa Miguel⁴

Affiliations

¹ Departament of Pathology, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
² Department of Cellular Biology and Genetics, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
³ Post-Graduation Program of Oral Pathology, Departament of Dentistry, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
⁴ Post-Graduation Program of Oral Pathology, Departament of Dentistry, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil. Electronic address: mccmiguel@hotmail.com.

PMID: 26742000
DOI: 10.1016/j.archoralbio.2015.12.004

Abstract

Objective: to evaluate the association between XPD and XRCC3 polymorphisms and oral squamous cell carcinoma (OSCC).

Design: the sample consisted of 54 cases of OSCC and 40 cases of inflammatory fibrous hyperplasia (IFH). Genotypes were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

Results: XPD-Lys/Gln was more common in IFH (n=28; 70%) than in OSCC (n=24; 44.4%) (OR: 0.3; p<0.05). XPD-Gln was more frequent in high-grade lesions (0.48) than in low-grade lesions (0.21) (OR: 3.4; p<0.05). The Gln/Gln genotype was associated with III and IV clinical stages (OR: 0.07; p<0.05). XRCC3-Met was more frequent in OSCC (0.49) than in IFH (0.35) (OR: 2.6; p<0.05). The Met/Met genotype was associated with the presence of metastases (OR: 8.1; p<0.05) and with III and IV clinical stages (OR: 0.07; p<0.05).

Conclusions: in this sample, the frequency of XPD-Gln in IFH suggests that this variant may protect against OSCC. The presence of the XRCC3-Met allele seems to contribute to the development of OSCC, metastases and more advanced stages in these lesions.

Keywords: DNA repair; Genetic polymorphisms; Oral cancer; Restriction fragment length polymorphisms; Squamous cell carcinoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brazil
Carcinoma, Squamous Cell / genetics*
Carcinoma, Squamous Cell / pathology
DNA Repair
DNA, Neoplasm / genetics
DNA, Neoplasm / isolation & purification
DNA-Binding Proteins / genetics*
Female
Gene Frequency
Genetic Predisposition to Disease
Genotype
Head and Neck Neoplasms / genetics*
Head and Neck Neoplasms / pathology
Humans
Male
Mouth Neoplasms / genetics*
Mouth Neoplasms / pathology
Neoplasm Metastasis
Pilot Projects
Polymerase Chain Reaction / methods
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Squamous Cell Carcinoma of Head and Neck
Xeroderma Pigmentosum Group D Protein / genetics*

Substances

DNA, Neoplasm
DNA-Binding Proteins
X-ray repair cross complementing protein 3
Xeroderma Pigmentosum Group D Protein
ERCC2 protein, human