ZYG11A serves as an oncogene in non-small cell lung cancer and influences CCNE1 expression

Xin Wang; Qi Sun; Chen Chen; Rong Yin; Xing Huang; Xuan Wang; Run Shi; Lin Xu; Binhui Ren

doi:10.18632/oncotarget.6904

ZYG11A serves as an oncogene in non-small cell lung cancer and influences CCNE1 expression

Oncotarget. 2016 Feb 16;7(7):8029-42. doi: 10.18632/oncotarget.6904.

Authors

Xin Wang^{1

2}, Qi Sun³, Chen Chen⁴, Rong Yin^{1

5}, Xing Huang^{1

2}, Xuan Wang², Run Shi^{1

2}, Lin Xu^{1

5}, Binhui Ren^{1

5}

Affiliations

¹ Department of Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Nanjing, Jiangsu, China.
² Department of The Fourth Clinical College, Nanjing Medical University, Nanjing, Jiangsu, China.
³ Department of Cardiothoracic Surgery at Jinling Hospital, Southern Medical University, Nanjing, Jiangsu, China.
⁴ Department of The Second Clinical College, Nanjing Medical University, Nanjing, Jiangsu, China.
⁵ Department of Thoracic Surgery, Jiangsu Cancer Hospital, Nanjing, Jiangsu, China.

Abstract

By analyzing The Cancer Genome Atlas (TCGA) database, we identified ZYG11A as a potential oncogene. We determined the expression of ZYG11A in NSCLC tissues and explored its clinical significance. And also evaluated the effects of ZYG11A on NSCLC cell proliferation, migration, and invasion both in vitro and in vivo. Our results show that ZYG11A is hyper-expressed in NSCLC tissues compared to adjacent normal tissues, and increased expression of ZYG11A is associated with a poor prognosis (HR: 2.489, 95%CI: 1.248-4.963, p = 0.010). ZYG11A knockdown induces cell cycle arrest and inhibits proliferation, migration, and invasion of NSCLC cells. ZYG11A knockdown also results in decreased expression of CCNE1. Over-expression of CCNE1 in cells with ZYG11A knockdown restores their oncogenic activities. Our data suggest that ZYG11A may serve as a novel oncogene promoting tumorigenicity of NSCLC cells by inducing cell cycle alterations and increasing CCNE1 expression.

Keywords: CCNE1; NSCLC; ZYG11A; bioinformatics; oncogene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / genetics
Adenocarcinoma / metabolism*
Adenocarcinoma / secondary
Apoptosis
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Blotting, Western
Carcinoma, Non-Small-Cell Lung / genetics
Carcinoma, Non-Small-Cell Lung / metabolism*
Carcinoma, Non-Small-Cell Lung / secondary
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism*
Carcinoma, Squamous Cell / secondary
Cell Cycle
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism*
Cell Movement
Cell Proliferation
Cyclin E / genetics
Cyclin E / metabolism*
Female
Flow Cytometry
Follow-Up Studies
Gene Expression Regulation, Neoplastic
Humans
Immunoenzyme Techniques
Lung Neoplasms / genetics
Lung Neoplasms / metabolism*
Lung Neoplasms / pathology
Lymphatic Metastasis
Male
Middle Aged
Neoplasm Grading
Neoplasm Staging
Oncogene Proteins / genetics
Oncogene Proteins / metabolism*
Oncogenes
Prognosis
RNA, Messenger / genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
Tumor Cells, Cultured

Substances

Biomarkers, Tumor
CCNE1 protein, human
Cell Cycle Proteins
Cyclin E
Oncogene Proteins
RNA, Messenger
ZYG11A protein, human