Knockdown of Lmo7 inhibits chick myogenesis

Ana C B Possidonio; Carolina P Soares; Marcio Fontenele; Eduardo R Morris; Vincent Mouly; Manoel L Costa; Claudia Mermelstein

doi:10.1002/1873-3468.12067

Knockdown of Lmo7 inhibits chick myogenesis

FEBS Lett. 2016 Feb;590(3):317-29. doi: 10.1002/1873-3468.12067. Epub 2016 Feb 1.

Authors

Ana C B Possidonio¹, Carolina P Soares¹, Marcio Fontenele², Eduardo R Morris¹, Vincent Mouly³, Manoel L Costa¹, Claudia Mermelstein¹

Affiliations

¹ Laboratório de Diferenciação Muscular e Citoesqueleto, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, RJ, Brazil.
² Laboratório de Biologia Molecular do Desenvolvimento, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, RJ, Brazil.
³ Sorbonne Universités, UPMC Univ Paris 06, INSERM UMRS974, CNRS FRE3617, Center for Research in Myology, Paris, France.

PMID: 26786059
DOI: 10.1002/1873-3468.12067

Abstract

The multifunctional protein Lmo7 has been implicated in some aspects of myogenesis in mammals. Here we studied the distribution and expression of Lmo7 and the effects of Lmo7 knockdown in primary cultures of chick skeletal muscle cells. Lmo7 was localized within the nuclei of myoblasts and at the perinuclear region of myotubes. Knockdown of Lmo7 using siRNA specific to chick reduces the number and width of myotubes and the number of MyoD positive-myoblasts. Both Wnt3a enriched medium and Bio, activators of the Wnt/beta-catenin pathway, could rescue the effects of the Lmo7 knockdown suggesting a crosstalk between the Wnt/beta-catenin and Lmo7-mediated signaling pathways. Our data shows a role of Lmo7 during the initial events of chick skeletal myogenesis, particularly in myoblast survival.

Keywords: Lmo7; MyoD; chick; muscle; myoblast; myogenesis; siRNA.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Avian Proteins / antagonists & inhibitors
Avian Proteins / genetics
Avian Proteins / metabolism*
Cell Nucleus / metabolism
Cell Nucleus / ultrastructure
Cells, Cultured
Chick Embryo
Cytoplasm / metabolism
Cytoplasm / ultrastructure
France
Green Fluorescent Proteins / antagonists & inhibitors
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
Humans
Infant, Newborn
LIM Domain Proteins / antagonists & inhibitors
LIM Domain Proteins / genetics
LIM Domain Proteins / metabolism*
Muscle Development*
Muscle Fibers, Skeletal / cytology
Muscle Fibers, Skeletal / metabolism*
Muscle Fibers, Skeletal / ultrastructure
Myoblasts, Skeletal / cytology
Myoblasts, Skeletal / metabolism*
Myoblasts, Skeletal / ultrastructure
Protein Transport
RNA Interference
RNA, Small Interfering
Recombinant Proteins / chemistry
Recombinant Proteins / metabolism
Sarcolemma / metabolism
Sarcolemma / ultrastructure
Transcription Factors / antagonists & inhibitors
Transcription Factors / genetics
Transcription Factors / metabolism*
Wnt Signaling Pathway

Substances

Avian Proteins
LIM Domain Proteins
LMO7 protein, human
RNA, Small Interfering
Recombinant Proteins
Transcription Factors
enhanced green fluorescent protein
Green Fluorescent Proteins