In Situ Hybridization of Breast Cancer Markers

Methods Mol Biol. 2016:1406:53-9. doi: 10.1007/978-1-4939-3444-7_4.

Abstract

In situ hybridization is an important technique in breast cancer research, which is widely applied in detection of specific nucleic acid sequences. Here, we describe the detailed protocol of fluorescence in situ hybridization and chromogenic in situ hybridization in detection of gene HER2/neu amplification in breast cancer tissues.

Keywords: Breast cancer; CISH; FISH; HER2; In situ hybridization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / genetics*
  • Breast Neoplasms / genetics*
  • Chromosomes, Human, Pair 17 / genetics
  • Gene Amplification
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Receptor, ErbB-2 / genetics

Substances

  • Biomarkers, Tumor
  • ERBB2 protein, human
  • Receptor, ErbB-2