Abstract
The activated form of the third complement protein, C3b, forms a stable complex with components of plasma membranes and particulate entities such as zymosan. The complex resists the action of detergents and protein denaturants as well as extremes of temperature, salt concentration, and pH. It can, however, be broken by exposure to hydroxylamine or by ammonolysis followed by incubation with sodium dodecyl sulfate. Thus, the complex appears to result from a hydrophobic interaction as well as a bond susceptible to nucleophilic attack.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Complement C3 / metabolism*
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Complement System Proteins / metabolism*
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Detergents / pharmacology
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Erythrocyte Membrane / metabolism*
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Erythrocytes / metabolism*
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Hydroxylamines / pharmacology
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Membrane Proteins / blood
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Membrane Proteins / metabolism
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Osmolar Concentration
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Protein Binding / drug effects
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Temperature
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Zymosan / metabolism*
Substances
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Complement C3
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Detergents
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Hydroxylamines
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Membrane Proteins
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Complement System Proteins
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Zymosan