Abstract
Non-small cell lung cancer (NSCLC) is one of the most common human malignancies, which threatens people's life heavily. Recently, TMEM98 is supposed to be of great value for the discoveries of anti-tumor drugs. We attempt to explore the biological role of TMEM98 in the human lung carcinoma. Clinical lung cancer tissue and normal tissue were collected, and the mRNA expression of TMEM98 in cancer tissue was significantly higher than that in normal tissue. By real-time-PCR and Western blot analysis of TMEM98 expression, human A549 and H460 cells were determined to carry out further investigations. By CCK8, it is found that siRNA-TMEM98 treatment effectively suppressed the proliferation of A549 and H460 cells. In addition, the invasion and migration of A549 and H460 cells were also inhibited by siRNA-TMEM98. We then studied the invasion and migration related proteins level by Western blot. From our result, the protein expression of MMP-2, MMP-9, RhoC and MTA1 were all regulated dramatically in siRNA-TMEM98 groups compared with the control and mock group. To conclude, our results indicated that siRNA-TMEM98 inhibited the invasion and migration of lung cancer cells, which can be considered as a novel target for NSCLC treatment.
Keywords:
TMEM98; invasion; lung cancer; migration.
MeSH terms
-
Carcinoma, Non-Small-Cell Lung / genetics*
-
Carcinoma, Non-Small-Cell Lung / metabolism
-
Carcinoma, Non-Small-Cell Lung / pathology
-
Cell Line, Tumor
-
Cell Movement*
-
Cell Proliferation
-
Gene Expression Regulation, Neoplastic
-
Histone Deacetylases / genetics
-
Histone Deacetylases / metabolism
-
Humans
-
Lung Neoplasms / genetics*
-
Lung Neoplasms / metabolism
-
Lung Neoplasms / pathology
-
Matrix Metalloproteinase 2 / genetics
-
Matrix Metalloproteinase 2 / metabolism
-
Matrix Metalloproteinase 9 / genetics
-
Matrix Metalloproteinase 9 / metabolism
-
Membrane Proteins / genetics*
-
Membrane Proteins / metabolism
-
Neoplasm Invasiveness
-
RNA Interference*
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism
-
RNA, Small Interfering / genetics*
-
RNA, Small Interfering / metabolism
-
Repressor Proteins / genetics
-
Repressor Proteins / metabolism
-
Signal Transduction
-
Time Factors
-
Trans-Activators
-
Transfection
-
rho GTP-Binding Proteins / genetics
-
rho GTP-Binding Proteins / metabolism
-
rhoC GTP-Binding Protein
Substances
-
Membrane Proteins
-
MTA1 protein, human
-
RNA, Messenger
-
RNA, Small Interfering
-
Repressor Proteins
-
TMEM98 protein, human
-
Trans-Activators
-
MMP2 protein, human
-
Matrix Metalloproteinase 2
-
MMP9 protein, human
-
Matrix Metalloproteinase 9
-
Histone Deacetylases
-
RHOC protein, human
-
rho GTP-Binding Proteins
-
rhoC GTP-Binding Protein