Beta3 adrenergic receptor is involved in vascular injury in deoxycorticosterone acetate-salt hypertensive mice

Li-Juan Sheng; Cheng-Chao Ruan; Yu Ma; Dong-Rui Chen; Ling-Ran Kong; Ding-Liang Zhu; Ping-Jin Gao

doi:10.1002/1873-3468.12107

Beta3 adrenergic receptor is involved in vascular injury in deoxycorticosterone acetate-salt hypertensive mice

FEBS Lett. 2016 Mar;590(6):769-78. doi: 10.1002/1873-3468.12107. Epub 2016 Mar 3.

Authors

Li-Juan Sheng¹, Cheng-Chao Ruan^{1

2}, Yu Ma¹, Dong-Rui Chen¹, Ling-Ran Kong¹, Ding-Liang Zhu¹, Ping-Jin Gao^{1

2}

Affiliations

¹ State Key Laboratory of Medical Genomics, Shanghai Key Laboratory of Hypertension and Department of Hypertension, Ruijin Hospital, Shanghai Institute of Hypertension, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
² Laboratory of Vascular Biology and Key Laboratory of Stem Cell Biology, Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China.

PMID: 26910302
DOI: 10.1002/1873-3468.12107

Abstract

Beta3 adrenergic receptor (ADRB3) mediates vessel relaxation in the endothelium while it modulates lipolysis in the adipose tissue. However, the function and regulation mechanism of ADRB3 in the perivascular adipose tissue (PVAT), especially in hypertension, is still unclear. We show that ADRB3 protein is upregulated in the PVAT of deoxycorticosterone acetate-salt (DOCA-salt) hypertensive mice, with the characteristics of PVAT browning and increased uncoupling protein 1 (UCP1) expression. Inhibition of ADRB3 with selective antagonist SR59230A caused serious vascular injury in vivo, even though UCP1 expression was downregulated. ADRB3 protein was regulated by let-7b, which was decreased in the PVAT of the DOCA-salt group. These data reveal that ADRB3 in PVAT contributes to vascular function in the progression of hypertension.

Keywords: DOCA-salt hypertensive mice; beta3 adrenergic receptor; hypertension; let-7b; perivascular adipose tissue; vascular injury.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

3' Untranslated Regions
3T3-L1 Cells
Adipose Tissue / blood supply
Adipose Tissue / drug effects
Adipose Tissue / metabolism
Adrenergic beta-3 Receptor Antagonists / pharmacology
Animals
Aorta, Thoracic / drug effects
Aorta, Thoracic / injuries
Aorta, Thoracic / metabolism
Base Sequence
Binding Sites / genetics
Cells, Cultured
Desoxycorticosterone Acetate
Humans
Hypertension / complications*
Hypertension / genetics
Hypertension / metabolism*
Ion Channels / metabolism
Male
Mice
Mice, Inbred C57BL
MicroRNAs / genetics
MicroRNAs / metabolism
Mitochondrial Proteins / metabolism
Molecular Sequence Data
Propanolamines / pharmacology
Receptors, Adrenergic, beta-3 / genetics
Receptors, Adrenergic, beta-3 / metabolism*
Uncoupling Protein 1
Vascular System Injuries / etiology*
Vascular System Injuries / genetics
Vascular System Injuries / metabolism*

Substances

3' Untranslated Regions
3-(2-ethylphenoxy)-1-(1,2,3,4-tetrahydronaphth-1-ylamino)-2-propanol oxalate
Adrenergic beta-3 Receptor Antagonists
Ion Channels
MicroRNAs
Mitochondrial Proteins
Propanolamines
Receptors, Adrenergic, beta-3
UCP1 protein, human
Ucp1 protein, mouse
Uncoupling Protein 1
mirnlet7 microRNA, mouse
Desoxycorticosterone Acetate