Prostate tumor overexpressed-1, in conjunction with human papillomavirus status, predicts outcome in early-stage human laryngeal squamous cell carcinoma

Lin Yang; Hongzhi Wang; Yan Wang; Zhenyu He; Haiyang Chen; Shaobo Liang; Shasha He; Shu Wu; Libing Song; Yong Chen

doi:10.18632/oncotarget.8103

Prostate tumor overexpressed-1, in conjunction with human papillomavirus status, predicts outcome in early-stage human laryngeal squamous cell carcinoma

Oncotarget. 2016 May 31;7(22):31878-91. doi: 10.18632/oncotarget.8103.

Authors

Lin Yang^{1

2

3}, Hongzhi Wang^{1

2

3}, Yan Wang^{1

2

3}, Zhenyu He^{1

2

3}, Haiyang Chen⁴, Shaobo Liang⁵, Shasha He^{1

2

3}, Shu Wu^{1

2

3}, Libing Song^{1

2

3}, Yong Chen^{1

2

3}

Affiliations

¹ Sun Yat-Sen University Cancer Center, Guangzhou, China.
² State Key Laboratory of Oncology in Southern China, Guangzhou, China.
³ Collaborative Innovation Center for Cancer Medicine, Guangzhou, China.
⁴ The Six Affiliated Hospital of Sun Yat-sen University, Guangzhou, China.
⁵ The First Hospital of Foshan, Foshan, China.

Abstract

In human cancer, molecular markers combined with clinical characteristics are used increasingly to predict prognosis. Prostate tumor overexpressed-1 (PTOV1), first identified in prostate cancer, is a key factor in tumor progression and correlates with unfavorable clinical outcomes. HPV infection status was tested by HPV E6-targeted multiplex real-time PCR and p16 immunohistochemistry (IHC). Real-time PCR and western blotting analyses were used to examine the mRNA and protein expression levels of PTOV1 in eight paired LSCC samples. IHC was performed to assess PTOV1 protein expression in 196 paraffin-embedded, archived LSCC samples. PTOV1 protein and mRNA expression was increased in LSCC tissues compared with adjacent non-cancerous tissue samples. High expression of PTOV1was significantly associated with advanced TNM stage by the χ2 test. Multivariate analysis revealed that PTOV1 and HPV status were independent prognostic indicators of overall survival (OS) and progression-free survival (PFS) (P = 0.001, P = 0.009 for OS, P = 0.005, P = 0.012 for PFS, respectively). Our study provides the first evidence that the combination of PTOV1 expression level and HPV status provides more prognostic information compared with HPV status alone with the significance still exists in the HPV negative subgroup.

Keywords: HPV; biomarker; laryngeal squamous cell carcinoma; prognosis; prostate tumor overexpressed-1.

Publication types

Comparative Study

MeSH terms

Adult
Biomarkers, Tumor / analysis*
Biomarkers, Tumor / genetics
Carcinoma, Squamous Cell / chemistry*
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / pathology
Carcinoma, Squamous Cell / virology*
Chi-Square Distribution
Cyclin-Dependent Kinase Inhibitor p16 / analysis
DNA, Viral / genetics
Disease Progression
Disease-Free Survival
Female
Head and Neck Neoplasms / chemistry*
Head and Neck Neoplasms / genetics
Head and Neck Neoplasms / pathology
Head and Neck Neoplasms / virology*
Human Papillomavirus DNA Tests
Humans
Immunohistochemistry
Kaplan-Meier Estimate
Laryngeal Neoplasms / chemistry*
Laryngeal Neoplasms / genetics
Laryngeal Neoplasms / pathology
Laryngeal Neoplasms / virology*
Male
Middle Aged
Multiplex Polymerase Chain Reaction
Multivariate Analysis
Neoplasm Proteins / analysis*
Neoplasm Proteins / genetics
Neoplasm Staging
Oncogene Proteins, Viral / analysis
Papillomaviridae / genetics
Papillomaviridae / isolation & purification*
Papillomavirus Infections / genetics
Papillomavirus Infections / pathology
Papillomavirus Infections / virology*
Predictive Value of Tests
Proportional Hazards Models
RNA, Messenger / genetics
Risk Factors
Squamous Cell Carcinoma of Head and Neck
Time Factors
Treatment Outcome

Substances

Biomarkers, Tumor
CDKN2A protein, human
Cyclin-Dependent Kinase Inhibitor p16
DNA, Viral
Neoplasm Proteins
Oncogene Proteins, Viral
PTOV1 protein, human
RNA, Messenger