Venturing into the New Science of Nucleases

Markéta Tolarová; John A McGrath; Jakub Tolar

doi:10.1016/j.jid.2016.01.021

Venturing into the New Science of Nucleases

J Invest Dermatol. 2016 Apr;136(4):742-745. doi: 10.1016/j.jid.2016.01.021.

Authors

Markéta Tolarová¹, John A McGrath², Jakub Tolar³

Affiliations

¹ Univerzita Karlova v Praze 2 lekarska fakulta, Medicine, Prague, Czech Republic.
² Genetic Skin Disease Group, St. John's Institute of Dermatology, London, UK.
³ Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota, USA. Electronic address: tolar003@umn.edu.

Abstract

Gene editing with zinc finger nucleases, transcription activator-like effector nucleases, clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated proteins system, or meganucleases can, in principle, mediate any genome modification. Recent studies have shown that COL7A1 mutations in cells of patients with recessive dystrophic epidermolysis bullosa can be corrected by homology-directed DNA repair.

Publication types

Comment

MeSH terms

Collagen Type VII / genetics*
DNA Repair*
Epidermolysis Bullosa Dystrophica / genetics*
Humans

Substances

Collagen Type VII

Abstract

Publication types

MeSH terms

Substances

Grants and funding