Dermal papilla cells (DPCs) are associated with development of hair follicles (HFs) and regulation of the hair cycle. However, primary DPCs are known to lose their ability to induce HFs after culture in standard media for fibroblasts. We examined a new culture condition for DPCs including addition of Wnt-10b, which promoted proliferation and maintained their HF induction ability for more than ten passages. These results suggest that Wnt-10b plays a pivotal role in proliferation and maintenance of DPCs in vitro.
Keywords: Cell culture; Cell proliferation; Dermal papilla; Wnt signaling; Wnt-10b.