Revelation of the IFNα, IL-10, IL-8 and IL-1β as promising biomarkers reflecting immuno-pathological mechanisms in porcine Huntington's disease model

Ivona Valekova; Karla Jarkovska; Eva Kotrcova; John Bucci; Zdenka Ellederova; Stefan Juhas; Jan Motlik; Suresh Jivan Gadher; Hana Kovarova

doi:10.1016/j.jneuroim.2016.02.012

Revelation of the IFNα, IL-10, IL-8 and IL-1β as promising biomarkers reflecting immuno-pathological mechanisms in porcine Huntington's disease model

J Neuroimmunol. 2016 Apr 15:293:71-81. doi: 10.1016/j.jneuroim.2016.02.012. Epub 2016 Feb 20.

Authors

Ivona Valekova¹, Karla Jarkovska², Eva Kotrcova¹, John Bucci³, Zdenka Ellederova², Stefan Juhas², Jan Motlik², Suresh Jivan Gadher⁴, Hana Kovarova⁵

Affiliations

¹ Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Vinicna 7, 128 43 Prague, Czech Republic.
² Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic.
³ Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, CA 92008, USA.
⁴ Thermo Fisher Scientific - Life Science Solutions, 7335 Executive Way, Frederick, MD 21704, USA.
⁵ Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, 277 21 Libechov, Czech Republic; Research Center PIGMOD, Rumburska 89, 277 21 Libechov, Czech Republic. Electronic address: kovarova@iapg.cas.cz.

PMID: 27049565
DOI: 10.1016/j.jneuroim.2016.02.012

Abstract

Studies on Huntington's disease (HD) demonstrated altered immune response in HD gene carriers. Using multiplexing immunoassay, we simultaneously investigated seven cytokines in secretomes of microglia and blood monocytes, cerebrospinal fluid (CSF) and serum collected from transgenic HD minipigs at pre-symptomatic disease stage. Decline in IFNα and IL-10 was observed in CSF and secretome of microglia whilst elevated IL-8 and IL-1β levels were secreted by microglia. Additionally, IL-8 was increased in serum. The proportion of mutant huntingtin in microglia may have causative impact on cytokine production. IFNα, IL-10, IL-8 and IL-1β represent promising biomarkers reflecting immuno-pathological mechanisms in porcine HD model.

Keywords: Biomarker(s); Central nervous system; Cytokines; Immune response; Porcine Huntington's disease model; Serum.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified
Biomarkers / metabolism
Calcium-Binding Proteins
Cells, Cultured
Central Nervous System / cytology
Central Nervous System / pathology
Cytokines / metabolism*
DNA-Binding Proteins / metabolism
Disease Models, Animal
Gene Expression Regulation / genetics
Humans
Huntingtin Protein
Huntington Disease / genetics
Huntington Disease / metabolism*
Huntington Disease / pathology*
Interferon-alpha / metabolism
Interleukin-10 / metabolism
Interleukin-1beta / metabolism
Interleukin-8 / metabolism
Lipopolysaccharides / pharmacology
Microfilament Proteins
Microglia / drug effects
Microglia / metabolism
Monocytes / drug effects
Monocytes / metabolism
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism
Swine
Swine, Miniature

Substances

AIF1 protein, human
Biomarkers
Calcium-Binding Proteins
Cytokines
DNA-Binding Proteins
HTT protein, human
Huntingtin Protein
Interferon-alpha
Interleukin-1beta
Interleukin-8
Lipopolysaccharides
Microfilament Proteins
Nerve Tissue Proteins
Interleukin-10