Panel of SEREX-defined antigens for breast cancer autoantibodies profile detection

Biomarkers. 2017 Mar;22(2):149-156. doi: 10.1080/1354750X.2016.1252952. Epub 2016 Nov 9.

Abstract

Content: Identification of panel of SEREX-defined antigens for breast cancer autoantibodies profile detection.

Objective: To create panel of antigens that can differentiate breast cancer patients and healthy individuals.

Methods: SEREX (serological analysis of cDNA expression libraries) method, ELISA (enzyme-linked immunosorbent assay), qPCR (quantitative polymerase chain reaction).

Results: In large-scale screening of 16 SEREX-antigens by sera of breast cancer patients and healthy donors, a combination of six antigens (RAD50, PARD3, SPP1, SAP30BP, NY-BR-62 and NY-CO-58) was identified, which can differentiate breast cancer patients and healthy donors with 70% sensitivity and 91% specificity. Elevated mRNA expression of SPP1 gene was revealed in breast tumors (2-7-fold) that correlated with SPP1 antigen immunoreactivity in autologous patients' sera.

Conclusions: The new panel of six SEREX-antigens was proposed, which enables creation of serological assay for breast cancer diagnostics and/or prognosis.

Keywords: Tumor-associated antigens; antigenic panel; autoantibody; breast cancer.

MeSH terms

  • Antigens, Neoplasm / blood*
  • Autoantibodies / analysis*
  • Breast Neoplasms / diagnosis*
  • Breast Neoplasms / immunology
  • Female
  • Humans
  • Osteopontin / genetics
  • Osteopontin / immunology
  • RNA, Messenger / analysis
  • Sensitivity and Specificity

Substances

  • Antigens, Neoplasm
  • Autoantibodies
  • RNA, Messenger
  • SPP1 protein, human
  • Osteopontin