Abstract
We have cloned a new species of rat Gi alpha (Gi3 alpha) cDNA and genomic DNAs for three distinct human Gi alpha proteins (Gi1 alpha, Gi2 alpha, and Gi3 alpha). Gi3 alpha cDNA codes for a protein of 354 amino acids (Mr 40,522) whose sequence is closely related but distinct from that of the previously isolated rat Gi alpha (Gi2 alpha). By screening the human genomic libraries with the two rat Gi alpha cDNAs as probes, clones encoding human Gi1 alpha, Gi2 alpha, and Gi3 alpha were isolated. The human Gi2 alpha and Gi3 alpha genes are composed of eight coding exons and seven introns and possess a completely identical exon-intron organization. Southern blot analysis indicates that a single copy of each Gi alpha gene is present per haploid human genome.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA / genetics*
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DNA Restriction Enzymes
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GTP-Binding Proteins / genetics*
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Genes*
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Humans
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Macromolecular Substances
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Molecular Sequence Data
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Nucleotide Mapping
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Rats
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Sequence Homology, Nucleic Acid
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Species Specificity
Substances
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Macromolecular Substances
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DNA
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DNA Restriction Enzymes
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GTP-Binding Proteins
Associated data
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GENBANK/J03219
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GENBANK/J03220
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GENBANK/J03221
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GENBANK/M19476
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GENBANK/M20586
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GENBANK/M20587
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GENBANK/M20588
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GENBANK/M20589
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GENBANK/M20590
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GENBANK/M20591
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GENBANK/M20592
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GENBANK/M20593
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GENBANK/M20594
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GENBANK/M20595
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GENBANK/M20596
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GENBANK/M20597
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GENBANK/M20598
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GENBANK/M20599
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GENBANK/M20600
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GENBANK/M20601
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GENBANK/M20602
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GENBANK/M20603
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GENBANK/M20604