Abstract
Myeloperoxidase gene transcription in isolated nuclei from HL-60 cells induced to differentiate into granulocytes by dimethyl sulfoxide or into macrophages by 12-O-tetradecanoylphorbol-13-acetate was studied by dot-blot hybridization of a myeloperoxidase cDNA to the 32P-labeled nuclear transcripts. Myeloperoxidase gene transcription, like that of c-myc gene transcription, was reduced to a low level within 12 h after the inductions of these differentiations. In contrast, transcription of the actin gene remained constant. These results indicate that decrease in myeloperoxidase synthesis in HL-60 cells during differentiation is regulated at a transcriptional level.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Differentiation / drug effects
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DNA / genetics
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Dimethyl Sulfoxide / pharmacology
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Enzyme Induction
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Granulocytes
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Humans
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Leukemia, Myeloid, Acute / enzymology
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Leukemia, Myeloid, Acute / pathology*
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Macrophages
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Male
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Middle Aged
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Neoplasm Proteins / biosynthesis*
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Peroxidase / biosynthesis*
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins c-myc
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RNA, Messenger / analysis
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RNA, Neoplasm / analysis
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Tetradecanoylphorbol Acetate / pharmacology
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Transcription, Genetic
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / enzymology
Substances
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Neoplasm Proteins
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-myc
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RNA, Messenger
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RNA, Neoplasm
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DNA
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Peroxidase
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Tetradecanoylphorbol Acetate
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Dimethyl Sulfoxide