The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism

Jing Tian; Jiapan Liu; Jieqiong Li; Jingxin Zheng; Lifang Chen; Yujuan Wang; Qiong Liu; Jiazuan Ni

doi:10.1186/s12986-017-0235-x

The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism

Nutr Metab (Lond). 2018 Jan 22:15:7. doi: 10.1186/s12986-017-0235-x. eCollection 2018.

Authors

Jing Tian¹, Jiapan Liu¹, Jieqiong Li², Jingxin Zheng³, Lifang Chen⁴, Yujuan Wang¹, Qiong Liu¹, Jiazuan Ni¹

Affiliations

¹ 1College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Marine Bioresources and Eco-environmental Science, Shenzhen University, Shenzhen, 518060 China.
² 2College of Life Sciences and Oceanography, Shenzhen Key Laboratory of Microbial Genetic Engineering, Shenzhen University, Shenzhen, 518060 China.
³ 3College of Life Sciences and Oceanography, Shenzhen Engineering Laboratory for Marine Algal Biotechnology, Shenzhen University, Shenzhen, 518060 China.
⁴ 4Department of Neurology, Shenzhen University 1st Affiliated Hospital, Shenzhen Second People's Hospital, Sungang West Road, Shenzhen, China.

Abstract

Background: Selenoprotein F (SELENOF, was named as 15-kDa selenoprotein) has been reported to play important roles in oxidative stress, endoplasmic reticulum (ER) stress and carcinogenesis. However, the biological function of SELENOF is still unclear.

Methods: A yeast two-hybrid system was used to screen the interactive protein of SELENOF in a human fetal brain cDNA library. The interaction between SELENOF and interactive protein was validated by fluorescence resonance energy transfer (FRET), co-immunoprecipitation (co-IP) and pull-down assays. The production of retinol was detected by high performance liquid chromatograph (HPLC).

Results: Retinol dehydrogenase 11 (RDH11) was found to interact with SELENOF. RDH11 is an enzyme for the reduction of all-trans-retinaldehyde to all-trans-retinol (vitamin A). The production of retinol was decreased by SELENOF overexpression, resulting in more retinaldehyde.

Conclusions: SELENOF interacts with RDH11 and blocks its enzyme activity to reduce all-trans-retinaldehyde.

Keywords: Co-immunoprecipitation (co-IP); Fluorescence resonance energy transfer (FRET); Protein-protein interaction; Pull-down; Retinaldehyde; Retinol (vitamin a); Retinol dehydrogenase 11 (RDH11); SELENOF (Seleonoprotein F); Yeast two hybrid system.