Knockout of NCOA5 impairs proliferation and migration of hepatocellular carcinoma cells by suppressing epithelial-to-mesenchymal transition

Biochem Biophys Res Commun. 2018 Jun 2;500(2):177-183. doi: 10.1016/j.bbrc.2018.04.017. Epub 2018 Apr 14.

Abstract

Nuclear receptor coactivator 5 (NCOA5) plays important roles in the development of a variety of malignancies. However, the underlying mechanisms remain obscure. In this study, we successfully generated the NCOA5 knockout hepatocellular carcinoma (HCC) cells by CRISPR/Cas9 - mediated genome editing and found that knockout of NCOA5 inhibited the proliferation and tumor microsphere formation of HCC cells significantly. Moreover, the migration ability of NCOA5 knockout HCC cells declined. Mechanistic analyses indicated that knockout of NCOA5 can suppress the epithelial - mesenchymal transition (EMT) in HCC cells. In conclusion, our findings provide a mechanistic insight into the role of NCOA5 in HCC progression.

Keywords: CRISPR/cas9; EMT; HCC; NCOA5.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • CRISPR-Cas Systems / genetics
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / pathology*
  • Cell Line, Tumor
  • Cell Movement* / genetics
  • Cell Proliferation / genetics
  • Epithelial-Mesenchymal Transition* / genetics
  • Gene Expression Regulation, Neoplastic
  • Gene Knockout Techniques*
  • Humans
  • Liver Neoplasms / genetics
  • Liver Neoplasms / pathology*
  • Nuclear Receptor Coactivators / metabolism*
  • Spheroids, Cellular / metabolism
  • Spheroids, Cellular / pathology

Substances

  • NCOA5 protein, human
  • Nuclear Receptor Coactivators