Abstract
We describe the basic principles of recombinant DNA technology and how this methodology has been used to isolate and characterise cloned genes coding for alpha 1-antitrypsin. We show how these probes can be used for diagnosis and to study molecular variants of alpha 1-antitrypsin which may predispose individuals to develop lung disease.
Publication types
-
Research Support, Non-U.S. Gov't
-
Review
MeSH terms
-
Amino Acids / analysis
-
Base Sequence
-
Chemical Phenomena
-
Chemistry
-
Cloning, Molecular
-
DNA
-
DNA Restriction Enzymes
-
DNA, Recombinant*
-
Genetic Markers
-
Humans
-
Lung Diseases / enzymology
-
Lung Diseases / genetics
-
Mutation
-
Risk
-
Smoking
-
alpha 1-Antitrypsin / genetics*
Substances
-
Amino Acids
-
DNA, Recombinant
-
Genetic Markers
-
alpha 1-Antitrypsin
-
DNA
-
DNA Restriction Enzymes