Cryo-EM structure of the polycystin 2-l1 ion channel

Elife. 2018 Jul 13:7:e36931. doi: 10.7554/eLife.36931.

Abstract

We report the near atomic resolution (3.3 Å) of the human polycystic kidney disease 2-like 1 (polycystin 2-l1) ion channel. Encoded by PKD2L1, polycystin 2-l1 is a calcium and monovalent cation-permeant ion channel in primary cilia and plasma membranes. The related primary cilium-specific polycystin-2 protein, encoded by PKD2, shares a high degree of sequence similarity, yet has distinct permeability characteristics. Here we show that these differences are reflected in the architecture of polycystin 2-l1.

Keywords: PC-2L1; TRP channel; calcium; cryo-EM; human; molecular biophysics; primary cilia; structural biology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium
  • Calcium Channels / chemistry
  • Calcium Channels / metabolism
  • Calcium Channels / ultrastructure*
  • Cations / metabolism
  • Cryoelectron Microscopy*
  • Humans
  • Models, Molecular
  • Protein Conformation
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / metabolism
  • Receptors, Cell Surface / ultrastructure*

Substances

  • Calcium Channels
  • Cations
  • PKD2L1 protein, human
  • Receptors, Cell Surface
  • Calcium