Abstract
DNAs from chronic granulocytic leukaemia (CGL) and chronic myelomonocytic leukaemia (CMML) were assayed for transforming genes by transfection into NIH 3T3 cells. Foci DNA was tagged with a geneticin-resistance cosmid, then followed through a drug selection and tumorigenicity assay. Activated Ha-ras genes, with point mutations at codon 12 (glycine to valine) were subsequently detected. The mutation was detected in the original samples by either MspI/HpaII digestion or polymerase chain reaction (PCR). Although mutations in the ras gene family may occur frequently in leukaemias, these are the first examples of Ha-ras mutations in CGL (blast crisis) and CMML.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blast Crisis / genetics*
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Blast Crisis / metabolism
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Cell Line
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Codon
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DNA, Neoplasm / genetics
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Gene Expression Regulation
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Humans
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics*
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / metabolism
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Leukemia, Myelomonocytic, Chronic / genetics*
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Leukemia, Myelomonocytic, Chronic / metabolism
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Mice
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Mutation
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics*
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Proto-Oncogene Proteins / biosynthesis
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins p21(ras)
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Transfection
Substances
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Codon
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DNA, Neoplasm
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Neoplasm Proteins
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Proto-Oncogene Proteins
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HRAS protein, human
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Proto-Oncogene Proteins p21(ras)