Spindlin docking protein (SPIN.DOC) interaction with SPIN1 (a histone code reader) regulates Wnt signaling

Indepth studies of protein-protein interactions are essential for discovering the molecular mechanisms and the biological context of protein functions. Even though previous study on the purification of SPIN1 interacting protein complex has shown Spindlin docking protein (SPIN.DOC) as the most abundant interacting protein partner; the study on the molecular function of SPIN.DOC is limited. Since the role of SPIN1 has been previously documented as a histone code reader and transcriptional coactivator of Wnt signaling, SPIN.DOC may probably involve in epigenetic regulation and Wnt signaling. This study aims to purify SPIN.DOC interacting protein complex and characterize the molecular function of SPIN.DOC. The finding of this study revealed that the suppression of SPIN.DOC expression in HEK293 cells by shRNA, slightly destabilized SPIN1 without any change in its chromatin localization. However, knockdown of SPIN1 decreased the expression and chromatin localization of SPIN.DOC. Nevertheless, overexpression of SPIN.DOC increased the expression and chromatin localization of SPIN1 but no change in the SPIN.DOC protein expression and chromatin localization when SPIN1 is overexpressed. TOPflash reporter assays revealed that SPIN.DOC regulates gene expression in Wnt signaling pathway and act as transcriptional repressor. Further, we show that C-terminal deleted mutant of SPIN.DOC is unable to interact with SPIN1. Unlike the wild type SPIN.DOC which acts as transcriptional repressor, overexpression of C-terminal deletion mutant activates Wnt signaling suggesting that SPIN.DOC-SPIN1 complex may act as transcriptional repressor. Overall, our data revealed new molecular functions of SPIN.DOC.