Selenoprotein SELENOK Enhances the Migration and Phagocytosis of Microglial Cells by Increasing the Cytosolic Free Ca²⁺ Level Resulted from the Up-Regulation of IP₃R

Enhancing the migration and phagocytosis of microglial cells is of great significance for the reducing of the risk of the neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD). The effect of mouse selenoprotein K (mSELENOK) on the migration and phagocytosis of BV2 microglial cells and its mechanism were studied. The results showed that the over-expression of mSELENOK can increase the migratory and phagocytic abilities of the microglial cells, while the knockdown of mSELENOK can decrease the migratory and phagocytic abilities of the cells. The cytosolic free Ca²⁺ level and inositol trisphosphate receptor (IP₃R) mRNA transcript and protein expression were also increased significantly as the consequence of the over-expression of mSELENOK in the microglial cells. On the contrary, the level of cytosolic free Ca²⁺ and the mRNA transcript and protein expression of IP₃R in mSELENOK knockdown cells were decreased significantly. 2-aminoethoxydiphenyl borate (2-APB), an antagonist of IP₃R, could prevent the increased migration, phagocytosis, and cytosolic free Ca²⁺ level of mSELENOK over-expressed microglial cells, and knockdown of IP₃R₃ could reduce the increased cytosolic Ca²⁺ level in mSELENOK over-expressed microglial cells. Further studies revealed that selenium supplement (Na₂SeO₃) can increase the expression of mSELENOK in microglial cells significantly. In summary, these data suggest that mSELENOK can increase cytosolic free Ca²⁺ level of microglial cells by up-regulating the expression of IP₃R, thus enhancing the migration and phagocytosis of microglial cells. Our results indicated that mSELENOK is an important selenoprotein, which plays a role in trace element selenium's functions and can enhance the migration and phagocytosis of microglial cells.