T-cell involvement in benign phase chronic myelogenous leukemia

Leuk Res. 1986;10(12):1433-9. doi: 10.1016/0145-2126(86)90010-x.

Abstract

T cells from the peripheral blood of patients with chronic myeloid leukemia (CML) were cultured with phytohemagglutinin and T-cell growth factor (TCGF) in agar culture. These T-cell colonies were pooled and expanded further in liquid culture with TCGF and then simultaneously analysed for the E-rosette receptor with the monoclonal antibody OKT11 and for the presence of the Philadelphia (Ph1) chromosome. OKT11 analysis showed these populations to be composed 99.5% or more of T cells. In four of the seven patients the T-cell suspension showed 7/50 (14%), 3/36 (8%), 2/34 (6%), and 4/44 (9%) Ph1 metaphases. Furthermore, Ph1 metaphases were demonstrated in T-cell cultures in two patients when bone marrow metaphases simultaneously showed 90 and 100% Ph1 negative metaphases secondary to human leukocyte interferon therapy or combination chemotherapy. A minority of T cells in benign phase CML have the Ph1 abnormality despite reduced number of Ph1 metaphases in bone marrow from therapy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface / analysis
  • Cells, Cultured
  • Female
  • Humans
  • Leukemia, Myeloid / etiology*
  • Leukemia, Myeloid / genetics
  • Male
  • Metaphase
  • Middle Aged
  • Philadelphia Chromosome
  • T-Lymphocytes / physiology*
  • T-Lymphocytes / ultrastructure

Substances

  • Antigens, Differentiation, T-Lymphocyte
  • Antigens, Surface