Transcriptional regulation of Bcl-2 gene by the PR/SET domain family member PRDM10

Na Chen; Taobo Hu; Yuanyuan Gui; Jieying Gao; Zhihong Li; Shi Huang

doi:10.7717/peerj.6941

Transcriptional regulation of Bcl-2 gene by the PR/SET domain family member PRDM10

PeerJ. 2019 May 15:7:e6941. doi: 10.7717/peerj.6941. eCollection 2019.

Authors

Na Chen¹, Taobo Hu², Yuanyuan Gui¹, Jieying Gao¹, Zhihong Li², Shi Huang¹

Affiliations

¹ Center for Medical Genetics, School of Life Sciences, Central South University, Changsha, Hunan, China.
² Department of Orthopedics, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.

Abstract

Bcl-2 (B-cell lymphoma 2) protein is localized in the outer membrane of mitochondria, where it plays an important role in promoting cellular survival and inhibiting the actions of pro-apoptotic proteins. PRDM10 is a member of the PR/SET family of epigenetic regulators and may play a role in development and cell differentiation. Here we show that human PRDM10 contributes to the transcriptional regulation of human Bcl-2 gene. We found that PRDM10-depletion in human cells reduced the expression of Bcl-2 protein and over-expression of PRDM10 promoted Bcl-2 protein expression. Furthermore, luciferase reporter activity of Bcl-2 gene P1 promoter was significantly increased in cells co-transfected with PRDM10, and PRDM10 was able to bind to the Bcl-2 P1 promoter in vivo. Using The Cancer Genome Atlas (TCGA) data set, we found weak positive correlation between PRDM10 and Bcl-2 in several cancer types including cancers of the breast, colon, and lung tissues. These data identify a novel function for PRDM10 protein and provide insights on the transcriptional control of Bcl-2 expression.

Keywords: B-cell lymphoma-2; Bcl-2; PRDM10 protein; The Cancer Genome Atlas; Transcription.

Grants and funding

This study was supported by the National Natural Science Foundation of China (81171880, 81330027, 81525007 and 31400919), the National Basic Research Program of China (2011CB51001, 2012CB517900), and the excellent youth science research program of the Education Department of Hunan Province (14B183). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.