Tripartite motif containing 14: An oncogene in papillary thyroid carcinoma

Wenyu Sun; Yunjun Wang; Duanshu Li; Yi Wu; Qinghai Ji; Tuanqi Sun

doi:10.1016/j.bbrc.2019.10.127

Tripartite motif containing 14: An oncogene in papillary thyroid carcinoma

Biochem Biophys Res Commun. 2020 Jan 8;521(2):360-367. doi: 10.1016/j.bbrc.2019.10.127. Epub 2019 Oct 24.

Authors

Wenyu Sun¹, Yunjun Wang¹, Duanshu Li¹, Yi Wu¹, Qinghai Ji¹, Tuanqi Sun²

Affiliations

¹ Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College of Fudan University, Shanghai, China.
² Department of Head and Neck Surgery, Fudan University Shanghai Cancer Center, Shanghai, China; Department of Oncology, Shanghai Medical College of Fudan University, Shanghai, China. Electronic address: Suntuanqi@shca.org.cn.

PMID: 31668806
DOI: 10.1016/j.bbrc.2019.10.127

Abstract

Tripartite motif (TRIM)-containing protein 14 (TRIM14) is implicated in many malignancies. Presently, we studied whether TRIM14 played a role in human papillary thyroid carcinoma (PTC). Herein, TRIM14 was up-regulated in tumor tissues when compared with normal thyroid samples. Among PTC patients, enhanced TRIM14 predicted short recurrence free survival (RFS) time. Then, we found that knockdown of TRIM14 in human PTC K1 cells inhibited cell proliferation, induced cell apoptosis and prevented the tumorigenicity in nude mice. On the contrary, TRIM14 overexpression in human PTC TPC1 cells promoted cell proliferation while inhibited cell apoptosis. TRIM14 exerted its oncogenic activities via promoting the activation of signal transducer and activator of transcription 3 (STAT3). Further, TRIM14 interacted with the suppressor of cytokine-signaling-1 (SOCS1), a negative regulator of STAT3 activation, and knockdown of TRIM14 inhibited SOCS1 ubiquitination. In conclusion, TRIM14 may be a prognostic factor and oncogene in PTC, and may be a potential target for PTC intervention.

Keywords: Papillary thyroid carcinoma (PTC); SOCS1; STAT3 activation; TRIM14.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis
Cell Line, Tumor
Cell Proliferation
Humans
Intracellular Signaling Peptides and Proteins / genetics*
Intracellular Signaling Peptides and Proteins / pharmacology
Mice
Mice, Nude
Oncogenes*
STAT3 Transcription Factor / metabolism
Suppressor of Cytokine Signaling 1 Protein / metabolism
Thyroid Cancer, Papillary / genetics*
Tripartite Motif Proteins / genetics*
Tripartite Motif Proteins / pharmacology
Ubiquitination / drug effects

Substances

Intracellular Signaling Peptides and Proteins
SOCS1 protein, human
STAT3 Transcription Factor
STAT3 protein, human
Suppressor of Cytokine Signaling 1 Protein
TRIM14 protein, human
Tripartite Motif Proteins