Effect arylamine N-acetyltransferase 1 on morphology, adhesion, migration, and invasion of MDA-MB-231 cells: role of matrix metalloproteinases and integrin αV

Cell Adh Migr. 2020 Dec;14(1):1-11. doi: 10.1080/19336918.2019.1710015.

Abstract

Reducted arylamine N-acetyltransferase (NAT1) in breast cancers is associated with poor patient survival. NAT1 has also been associated with changes in cancer cell survival and invasion both invitro and invivo. Here, we report the effects of NAT1 in cancer cell invasion by addressing its role in adherence, migration, and invasion in vitro. The NAT1 gene was deleted in MDA-MB-231, HT-29 and HeLa cells using CRISPR/Cas9 gene editing. Loss of NAT1 increased adherence to collagen in all three cell-lines but migration was unaffected. NAT1 deletion decreased invasion and induced changes to cell morphology. These effects were independent of matrix metalloproteinases but were related to integrin ITGαV expression. The data suggest NAT1 is important in adhesion and invasion through integrin expression.

Keywords: NAT1; adherence; arylamine; integrin. MMP9; invasion; migration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arylamine N-Acetyltransferase / metabolism*
  • Cell Adhesion
  • Cell Line, Tumor
  • Cell Movement*
  • Cell Shape*
  • Gene Deletion
  • Humans
  • Integrin alphaV / metabolism*
  • Isoenzymes / metabolism*
  • Matrix Metalloproteinases / metabolism*
  • Neoplasm Invasiveness

Substances

  • Integrin alphaV
  • Isoenzymes
  • Arylamine N-Acetyltransferase
  • N-acetyltransferase 1
  • Matrix Metalloproteinases

Grants and funding

This work was funded by the National Health and Medical Research Council [Grant number 1083036]. Pengcheng Li was supported by the CSC-UQ PhD Scholarship program;National Health and Medical Research Council [1083036].