In this study, we investigated the role of XIST in the development of diabetic nephropathy (DN) and further explored its underlying mechanism. qRT-PCR was used to examine the level of XIST in serum of DN patients. ELISA, MTT, and flow cytometry were used to investigate the effect of XIST on biological functions of human mesangial cells (HMCs) treated with high glucose. The recovery experiments were used to explore the potential mechanism. The result showed XIST expression was elevated significantly in serums of DN patients. XIST silencing alleviated the induction of high glucose in biological behaviour of HMCs. Besides, miR-485 inhibitor revised the suppression by si-XIST in biological behaviour of high glucose induced HMCs. Furthermore, PSMB8 mimic relieved the inhibition of si-XIST in biological behaviour of high glucose induced HMCs. In short, XIST silencing could alleviate biological process and inflammation of HMCs treated with high glucose by sponging miR-485.
Keywords: Diabetic nephropathy; PSMB8; XIST; miR-485.