Epidermal growth factor (EGF) receptor gene transcription. Requirement for Sp1 and an EGF receptor-specific factor

J Biol Chem. 1988 May 5;263(13):6329-36.

Abstract

We have studied in vitro transcription of the human epidermal growth factor (EGF) receptor proto-oncogene using nuclear extracts of A431 human epidermoid carcinoma cells, which overproduce the EGF receptor. With the in vitro system we found that Sp1 and other trans-acting factors bound to the EGF receptor promoter regions and are required for maximal expression. Fractionation showed that a DEAE-Sepharose fraction (BA) contained a novel factor, which specifically stimulated EGF receptor transcription 5- to 10-fold. The molecular mass of the native form of the factor is about 270-kDa based on its migration on Sephacryl S-300. This factor may activate transcription of the proto-oncogene through a weak or indirect interaction with the DNA template.

MeSH terms

  • Base Sequence
  • Carcinoma, Squamous Cell / genetics
  • Cell Line
  • Chromosome Deletion
  • DNA-Binding Proteins / metabolism*
  • Deoxyribonuclease I / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • ErbB Receptors / genetics*
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Mutation
  • Promoter Regions, Genetic
  • Proto-Oncogene Mas
  • Proto-Oncogenes
  • Sp1 Transcription Factor
  • Transcription Factors / metabolism*
  • Transcription, Genetic*

Substances

  • DNA-Binding Proteins
  • MAS1 protein, human
  • Proto-Oncogene Mas
  • Sp1 Transcription Factor
  • Transcription Factors
  • ErbB Receptors
  • Deoxyribonuclease I

Associated data

  • GENBANK/J03206