Carboxyl terminus of Hsc70-interacting protein (CHIP) promotes pulmonary artery smooth muscle cell (PASMC) proliferation via enhancement of intracellular Ca2+ concentration ([Ca2+]i)

Exp Lung Res. 2020 Nov;46(9):332-340. doi: 10.1080/01902148.2020.1781296. Epub 2020 Sep 2.

Abstract

To investigate the effect of carboxyl terminus of Hsc70-interacting protein (CHIP) on pulmonary arterial smooth muscle cell (PASMC) proliferation and the underlying mechanism. Materials and Methods: PASMCs were harvested from distal PAs isolated from SD rat lungs and cultured. After CHIP overexpression, PASMCs were exposed to normoxia or hypoxia for 60 h. Then, PASMC proliferation, store-operated Ca2+ entry (SOCE), [Ca2+]i and the expression of TRPC1, TRPC4, and TRPC6 in PASMCs were measured. Results: CHIP overexpression promoted PASMC proliferation, SOCE, [Ca2+]i and the expression of TRPC1, TRPC4, and TRPC6. Conclusions: CHIP stimulates PASMC proliferation likely by targeting the TRPC1,4,6-SOCE-[Ca2+]i signaling pathway.

Keywords: CHIP; PASMCs; SOCE; TRPC; [Ca2+]i.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Cell Proliferation / physiology*
  • Cells, Cultured
  • HSC70 Heat-Shock Proteins / metabolism*
  • Hypertension, Pulmonary / metabolism
  • Hypoxia / metabolism
  • Muscle, Smooth, Vascular / metabolism*
  • Myocytes, Smooth Muscle / metabolism*
  • Pulmonary Artery / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Signal Transduction / physiology
  • TRPC Cation Channels / metabolism

Substances

  • HSC70 Heat-Shock Proteins
  • Hspa8 protein, rat
  • TRPC Cation Channels
  • Calcium