Methyl-CpG binding domain protein 3: a new diagnostic marker and potential therapeutic target of melanoma

Takayuki Ishibashi; Ikko Kajihara; Satoru Mizuhashi; Haruka Kuriyama; Toshihiro Kimura; Hisashi Kanemaru; Katsunari Makino; Azusa Miyashita; Jun Aoi; Takamitsu Makino; Satoshi Fukushima; Kanako Kita; Hironobu Ihn

doi:10.5582/bst.2020.01048

Methyl-CpG binding domain protein 3: a new diagnostic marker and potential therapeutic target of melanoma

Biosci Trends. 2020 Nov 4;14(5):390-395. doi: 10.5582/bst.2020.01048. Epub 2020 Sep 18.

Affiliations

¹ Department of Dermatology and Plastic Surgery, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.
² Department of Molecular Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan.

PMID: 32963182
DOI: 10.5582/bst.2020.01048

Abstract

Methyl-CpG binding domain protein 3 (MBD3) belongs to the methyl-CpG binding protein family. MBD3 facilitates the initiation of neural stem cell reprogramming. Melanoma originates in melanocytes derived from neural crest stem cells; therefore, we investigated the role of MBD3 in melanoma. MBD3 was overexpressed in melanoma compared with pigmented nevi. MBD3 knockdown had no effect on the proliferation of melanoma cells (A375 and A2058 cells). Contrarily, it significantly reduced the migration and invasion of A375 cells, but had no significant effect on A2058 cells. Furthermore, MBD3 knockdown reduced N-cadherin protein levels and matrix metalloproteinase-2 (MMP-2) activity in A375 cells, but had no significant effect on A2058 cells. Based on these results, the MBD3 expression level may be a useful biomarker for the diagnosis of melanoma. Thus, MBD3 has potential as a novel therapeutic target for some melanoma patients.

Keywords: MBD3; MMP-2; N-cadherin; melanoma.

Publication types

Comparative Study
Observational Study

MeSH terms

Adult
Aged
Aged, 80 and over
Antineoplastic Agents / pharmacology
Antineoplastic Agents / therapeutic use
Biomarkers, Tumor / analysis
Biomarkers, Tumor / antagonists & inhibitors
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Cell Line, Tumor
Cell Movement / genetics
DNA-Binding Proteins / analysis
DNA-Binding Proteins / antagonists & inhibitors
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Diagnosis, Differential
Epigenesis, Genetic / drug effects
Female
Gene Knockdown Techniques
Humans
Male
Melanoma / diagnosis*
Melanoma / drug therapy
Melanoma / pathology
Middle Aged
Neoplasm Invasiveness / genetics
Neoplasm Invasiveness / pathology
Nevus, Pigmented / diagnosis*
Nevus, Pigmented / drug therapy
Nevus, Pigmented / pathology
Skin / pathology
Skin Neoplasms / diagnosis*
Skin Neoplasms / drug therapy
Skin Neoplasms / pathology

Substances

Antineoplastic Agents
Biomarkers, Tumor
DNA-Binding Proteins
MBD3 protein, human